Human Progesterone R B/NR3C3 B Antibody

Catalog #: PP-H5344-00 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
PP-H5344-00
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Human Progesterone R B/NR3C3 B Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically reacts with human PR isoform B and cross-reacts with mouse and rat PR isoform B. It does not cross-react with human PR isoform A. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # H5344
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human Progesterone R B/NR3C3
aa 2-99
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL under reducingconditions and at 3 μg/mL under non-reducing conditions with the appropriatesecondary reagents to detect human PR Isoform B.
 
Immunohistochemistry
This antibody can be used at 10 - 20 μg/mL withthe appropriate secondary reagents to detect human PR Isoform B.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 0.3 μg/mL with the appropriatesecondary reagents to detect human PR Isoform B.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Progesterone R B/NR3C3

Progesterone Receptor (PR; NR3C3) is a member of the Steroid Receptor superfamily. PR binds Progesterone. It is expressed in the uterus, ovary, vagina, fallopian tubes and breast. PR plays a central role in diverse reproductive events associated with the establishment and maintenance of pregnancy, alveolar development in the breast, and sexual behavior. Two isoforms of this receptor have been identified, PR-A and PR-B, 94 and 114 kDa in size, respectively. It has been shown that PR-A and PR-B isoforms can form homodimers as well as heterodimers.

Long Name
Progesterone Receptor B
Entrez Gene IDs
5241 (Human)
Alternate Names
PGRB; Progesterone R B; Progesterone RB

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