Human PSMA/FOLH1/NAALADase I Antibody Summary
Lys44-Ala750
Accession # Q04609
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human NAALADase I by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB4234) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NAALADase I at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of NAALADase I in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB4234, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
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NAALADase I in PC‑3 Human Cell Line. NAALADase I was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human PSMA/FOLH1/ NAALADase I Monoclonal Antibody (Catalog # MAB4234) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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NAALADase I in Human Prostate. NAALADase I was detected in formalin fixed paraffin-embedded sections of human prostate using Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB4234) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSMA/FOLH1/NAALADase I
Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).
- Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
- Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
- Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
- Heston, W.D. (1997) Urology 49:104.
Product Datasheets
Citations for Human PSMA/FOLH1/NAALADase I Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Improved Prostate-Specific Membrane Antigen (PSMA) Stimulation Using a Super Additive Effect of Dutasteride and Lovastatin In Vitro
Authors: Kuzmanov, A;Salemi, S;Schmid, FA;Burger, IA;Eberli, D;Kranzbühler, B;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Inhibition of glutamate-carboxypeptidase-II in dorsolateral prefrontal cortex: potential therapeutic target for neuroinflammatory cognitive disorders
Authors: S Yang, D Datta, Elizabeth, A Duque, YM Morozov, J Arellano, BS Slusher, M Wang, AFT Arnsten
Molecular Psychiatry, 2022-06-22;0(0):.
Species: Rhesus Macaque
Sample Types: Whole Tissue
Applications: IHC -
Identification of epithelial and mesenchymal circulating tumor cells in clonal lineage of an aggressive prostate cancer case
Authors: Shoujie Chai, Carmen Ruiz-Velasco, Amin Naghdloo, Milind Pore, Mohan Singh, Nicholas Matsumoto et al.
npj Precision Oncology
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Glutamate Carboxypeptidase II in Aging Rat Prefrontal Cortex Impairs Working Memory Performance
Authors: Dibyadeep Datta, Shannon N. Leslie, Elizabeth Woo, Nishita Amancharla, Ayah Elmansy, Miguel Lepe et al.
Frontiers in Aging Neuroscience
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