Human PTH1R/PTHR1 Antibody

Catalog #: MAB57091 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB57091-100
MAB57091-SP

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Human PTH1R/PTHR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PTHR1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 542407

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human PTHR1
Tyr23-Met189
Accession # Q03431

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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PTH1R/PTHR1 in Human Kidney. PTH1R/PTHR1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human PTH1R/PTHR1 Monoclonal Antibody (Catalog # MAB57091) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTH1R/PTHR1

PTH1R (Parathyroid hormone receptor # 1; also PTH/PTHr receptor) is a predicted 66-68 kDa member of the G-protein coupled receptor family # 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca⁺⁺-release from bone, mediated by osteoclast formation. Mature human PTH1R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1R signaling. Over aa 1-189, human PTH1R shares 88% aa identity with mouse PTH1R.

Long Name

Parathyroid Hormone Receptor 1

Entrez Gene IDs

5745 (Human); 19228 (Mouse); 56813 (Rat)

Alternate Names

MGC138426; parathyroid hormone 1 receptorMGC138452; parathyroid hormone/parathyroid hormone-related peptide receptor; parathyroid hormone/parathyroid hormone-related protein receptor; PFE; PTH/PTHr receptor; PTH/PTHrP type I receptor; PTH1 receptor; PTH1R; PTHR; PTHR1; PTHRPTHR1parathyroid hormone receptor 1; seven transmembrane helix receptor