Human PTPN14 Antibody Summary
Leu329-Leu900
Accession # Q15678.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PTPN14 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPN14 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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PTPN14 in Human Placenta. PTPN14 was detected in immersion fixed paraffin-embedded sections of human placenta using Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTPN14
Protein Tyrosine Phosphatase, Non-receptor type 14 (PTPN14), also called Phosphatase with Ezrin Domain (PEZ) and PTP36, is a 135 kDa cytosolic protein that dephosphorylates tyrosine residues in proteins. PTPN14’s Ezrin domain is homologous to erythrocyte Band 4.1 protein, targeting the phosphatase to cytoskeletal elements such as actin. PTPN14 associates with beta-catenin in adhesion zones in cells that are touching, but is nuclear in isolated, proliferating cells. Loss-of-function mutations in PTPN14 are found in 26% of colorectal cancers, suggesting it is a tumor suppressor.
Product Datasheets
Citations for Human PTPN14 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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The Tyrosine Phosphatase PTPN14 Is a Negative Regulator of YAP Activity
Authors: Chrysiis Michaloglou, Waltraut Lehmann, Typhaine Martin, Clara Delaunay, Andreas Hueber, Louise Barys et al.
PLoS ONE
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The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell–cell adhesion
Authors: Gareth W Fearnley, Katherine A Young, James R Edgar, Robin Antrobus, Iain M Hay, Wei-Ching Liang et al.
eLife
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A proximity proteomics screen in three-dimensional spheroid cultures identifies novel regulators of lumen formation
Authors: LT Wang, MÈ Proulx, AD Kim, V Lelarge, L McCaffrey
Scientific Reports, 2021-11-23;11(1):22807.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: IHC, Western Blot -
The non-receptor tyrosine phosphatase type 14 blocks caveolin-1-enhanced cancer cell metastasis
Authors: NI Díaz-Valdi, J Díaz, P Contreras, A Campos, V Rojas-Celi, RA Burgos-Rav, L Lobos-Gonz, VA Torres, VI Perez, B Frei, L Leyton, AFG Quest
Oncogene, 2020-03-09;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The effect of YAP expression in tumor cells and tumor stroma on the prognosis of patients with squamous cell carcinoma of the oral cavity floor and oral surface of the tongue
Authors: J Szelachows, P Donizy, K Ratajczak-, A Halon, D Zielecka-D, K Lichon, A Maciejczyk, E Lata-Wozni, A Piotrowska, R Matkowski
Oncol Lett, 2019-07-31;18(4):3561-3570.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Protein-tyrosine Phosphatase and Kinase Specificity in Regulation of SRC and Breast Tumor Kinase.
Authors: Fan G, Aleem S, Yang M, Miller W, Tonks N
J Biol Chem, 2015-04-20;290(26):15934-47.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A novel phosphatidic acid-protein-tyrosine phosphatase D2 axis is essential for ERBB2 signaling in mammary epithelial cells.
Authors: Ramesh M, Krishnan N, Muthuswamy S, Tonks N
J Biol Chem, 2015-02-13;290(15):9646-59.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protein-tyrosine phosphatase 1B antagonized signaling by insulin-like growth factor-1 receptor and kinase BRK/PTK6 in ovarian cancer cells.
Authors: Fan G, Lin G, Lucito R, Tonks N
J Biol Chem, 2013-06-28;288(34):24923-34.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
YAP modifies cancer cell sensitivity to EGFR and survivin inhibitors and is negatively regulated by the non-receptor type protein tyrosine phosphatase 14.
Authors: Huang JM, Nagatomo I
2012-06-11;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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