Human Ras Antibody

Catalog #: MAB3429
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3429-100
MAB3429-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ras Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ras (H-Ras, N-Ras, and K-Ras) in Western blots. Does not detect more distantly related Ras superfamily members, including R-Ras, Ral, Rap, and Rheb.

Source

Monoclonal Rat IgG_2B Clone # 342404

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human K-Ras2B
Met1-Met188 (Gln61His)
Accession # P01116-2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Ras by Western Blot. Western blot shows lysates of K562 human chronic myelo-genous leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Human Ras Monoclonal Antibody (Catalog # MAB3429) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional refer-ence, recombinant human H-Ras, N Ras, K-Ras2A and 2B, R-Ras2 and 3, Rap2A and 2B, RalA and B, and Rheb (5 ng/lane) were included. A specific band for Ras was detected at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ras

The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.

Long Name

v-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Jomolog

Entrez Gene IDs

3845 (Human); 16653 (Mouse); 24525 (Rat)

Alternate Names

c-K-ras; GTPase HRas; GTPase NRas; Harvey rat sarcoma viral oncogene homolog; HRAS; HRAS1; Ki-Ras; KRAS; KRAS1; KRAS2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; N-ras; NRAS1; Oncogene Ras; proto-oncogene GTPase; Ras family small GTP binding protein H-Ras; Ras; RASK2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human Ras Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence
Authors: V Farfariell, DV Gordienko, L Mesilmany, Y Touil, E Germain, I Fliniaux, E Desruelles, D Gkika, M Roudbaraki, G Shapovalov, L Noyer, M Lebas, L Allart, N Zienthal-G, O Iamshanova, F Bonardi, M Figeac, W Laine, J Kluza, P Marchetti, B Quesnel, D Metzger, D Bernard, JB Parys, L Lemonnier, N Prevarskay
Nature Communications, 2022-02-17;13(1):956.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot