Key Product Details

Species Reactivity

Validated:

Human, Rat, Chicken

Cited:

Human, Rat

Applications

Validated:

Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C4
View all Laminin S Primary Antibodies »

Product Specifications

Immunogen

Bovine anterior lens capsule

Specificity

Detects human, rat, and chicken Laminin S in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Rat/Chicken Laminin S Antibody

Laminin S antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Laminin S in HeLa Human Cell Line.

Laminin S was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Rat/Chicken Laminin S Monoclonal Antibody (Catalog # MAB2066) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat/Chicken Laminin S Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LS180 human colorectal adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Immunoprecipitation

Green, T.L. et al. (1992) J. Biol. Chem. 267:2014.

Western Blot

Green, T.L. et al. (1992) J. Biol. Chem. 267:2014. This application was not tested by R&D Systems.

Reviewed Applications

Read 1 review rated 5 using MAB2066 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Laminin S

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins critically contribute to cell differentation, cell shape and migration and maintenance of tissue phenotypes and survival (1). Laminin S, also known as laminin beta 2, is a subunit of laminin-3 (S-laminin), laminin-4 (S-merosin) and laminin-7 (KS-laminin). It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle.

References

  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.

Long Name

Laminin, beta 2

Alternate Names

LAMB2, Laminin beta 2

Entrez Gene IDs

3913 (Human)

Gene Symbol

LAMB2

Additional Laminin S Products

Product Documents for Human/Rat/Chicken Laminin S Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Rat/Chicken Laminin S Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Human/Rat/Chicken Laminin S Antibody

Customer Reviews for Human/Rat/Chicken Laminin S Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Rat/Chicken Laminin S Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human/Rat/Chicken Laminin S Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 05/18/2022
    Human/Rat/Chicken Laminin S Antibody MAB2066

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies