Human RBP4/Retinol-Binding Protein 4 Antibody

Catalog #: MAB33781 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB33781-SP
MAB33781-500
MAB33781-100

All RBP4/Retinol-Binding Protein 4 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RBP4/Retinol-Binding Protein 4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RBP4/Retinol‑Binding Protein 4 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse RBP4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 393006

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human RBP4/Retinol‑Binding Protein 4
Glu19-Leu201
Accession # P02753

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Human RBP4/Retinol Binding Protein 4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human RBP4/Retinol‑Binding Protein 4 Biotinylated Antibody (Catalog # BAM33782)

Standard: Recombinant Human RBP4 Protein, CF (Catalog # 3378-LC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution (1, 2). However, retinol becomes quite stable and soluble in plasma due to its tight interaction with retinol-binding protein 4 (RBP4), also known as plasma retinol-binding protein. A prototypic member of the lipocalin superfamily, RBP4 has a beta -barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision. Human and mouse RBP4 share 86% amino acid sequence identity.

References