Human Reg4 Antibody Summary
Asp23-Pro158
Accession # Q9BYZ8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Reg4 in Human Colon Cancer Tissue. Reg4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Reg4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1379) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg4
Reg4 (regenerating islet-derived protein 4; also named Reg-like protein, RELP, and gastrointestinal secretory protein) is a 17 kDa, secreted glycoprotein belonging to the calcium (C-type) dependent lectin superfamily and the regenerating gene family (1-3). There are three potential isoforms for human Reg4. Isoform 1 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain. Amino acids 30-156 constitute a C-type lectin-like domain, and amino acid 50 is a site for potential N-linked glycosylation. In addition, amino acids 127 and 142-143 form a ligand-binding surface. In isoform 2, there is a 79 aa substitution for the C-terminal 101 amino acids, while in isoform 3, there is an 11 aa substitution for the C-terminal 57 amino acids. Human Reg4 shares 68% and 67% aa sequence identity with rat and mouse Reg4, respectively. Like other members of the regenerating gene family, Reg4 is expressed in the gastrointestinal (GI) tract and ectopically at other sites in the setting of tissue injury (1, 2). In particular, Reg4 is expressed in normal colon mucosa, and up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, inflammatory bowel disease (Crohn’s disease and ulcerative colitis), and, outside the GI tract, in prostate adenocarcinoma (2-8). The physiological function of Reg4 is presently unknown, but it may be involved in inflammatory and metaplastic responses of the GI epithelium (3).
- Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
- Zhang, Y. et al. (2003) World J. Gastroenterol. 9:2635.
- Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
- Zhang, Y. et al. (2003) Cancer Lett. 200:69.
- Violette, S. et al. (2003) Int. J. Cancer 103:185.
- Oue, N. et al. (2005) J. Pathol. 207:185.
- Zhennan, G. et al. (2005) Clin. Cancer Res. 2:2237
- Bishnupuri, K.S. et al. (2006) Gastroenterology 130:137.
Product Datasheets
Citations for Human Reg4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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REG gene expression in inflamed and healthy colon mucosa explored by in situ hybridisation.
Authors: van Beelen Granlund A, Ostvik AE, Brenna O et al.
Cell Tissue Res
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REG4 Is Highly Expressed in Mucinous Ovarian Cancer: A Potential Novel Serum Biomarker
Authors: Laura Lehtinen, Pia Vesterkvist, Pia Roering, Taina Korpela, Liisa Hattara, Katja Kaipio et al.
PLOS ONE
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ARTS mediates apoptosis and regeneration of the intestinal stem cell niche
Authors: E Koren, Y Yosefzon, R Ankawa, D Soteriou, A Jacob, A Nevelsky, R Ben-Yosef, G Bar-Sela, Y Fuchs
Nat Commun, 2018-11-02;9(1):4582.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
IL2RG, identified as overexpressed by RNA-seq profiling of pancreatic intraepithelial neoplasia, mediates pancreatic cancer growth
Authors: M Ayars, E O'Sullivan, A Macgregor-, K Shindo, H Kim, M Borges, J Yu, RH Hruban, M Goggins
Oncotarget, 2017-08-03;8(48):83370-83383.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon
Proc Natl Acad Sci USA, 2016-08-29;113(37):E5399-407.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
RNA sequencing of sessile serrated colon polyps identifies differentially expressed genes and immunohistochemical markers.
Authors: Delker D, McGettigan B, Kanth P, Pop S, Neklason D, Bronner M, Burt R, Hagedorn C
PLoS ONE, 2014-02-12;9(2):e88367.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
REG4 acts as a mitogenic, motility and pro-invasive factor for colon cancer cells.
Authors: Rafa L, Dessein AF, Devisme L, Buob D, Truant S, Porchet N, Huet G, Buisine MP, Lesuffleur T
Int. J. Oncol., 2010-03-01;36(3):689-98.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Reg IV is a serum biomarker for gastric cancer patients and predicts response to 5-fluorouracil-based chemotherapy.
Authors: Mitani Y, Oue N, Matsumura S, Yoshida K, Noguchi T, Ito M, Tanaka S, Kuniyasu H, Kamata N, Yasui W
Oncogene, 2007-01-22;26(30):4383-93.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Activation of REG family proteins in colitis.
Authors: Granlund Av, Beisvag V, Torp SH et al.
Scand J Gastroenterol
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We used this antibody for a sandwich ELISA format in combination with mAb (1379) and protein (1379-RG). This combination worked very well and detected human Reg 4 in serum and plasma samples.
For immunostaining to make unique populations of secretory progenitor cells in intestinal stem cell-derived organoid cultures.
