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Human ROBO1 Antibody

Catalog #: AF7118
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7118
AF7118-SP
Detection of Human ROBO1 by Western Blot.
2 Images
Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROBO1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROBO1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat ROBO1 is observed, and less than 3% cross-reactivity with recombinant human (rh) ROBO2 and rhROBO3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO1
Ser20-Pro861 (Val310-Ser312 del)
Accession # NP_598334
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ROBO1 antibody by Western Blot. View Larger

Detection of Human ROBO1 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7118) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ROBO1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ROBO1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

ROBO1 in HepG2 Human Cell Line. ROBO1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7118) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO1

ROBO1 (Roundabout-like protein 1) is a 190-230 kDa member of the ROBO/roundabout receptor family. It is expressed by commissural axons from multiple nuclei, and is also found on vascular endothelium, bronchial epithelium, and synctiotrophoblasts. It interacts with Slit and DCC to temporally regulate the migration of axonal processes. The human ROBO1 precursor is a 1651 amino acid (aa) type I transmembrane protein. It contains a 25 aa signal sequence, followed by an 872 aa extracellular region (aa 26-897) that possesses five C2-type Ig-like domains (aa 68-541) and three fibronectin type III domains (aa 561-864) (SwissProt # Q9Y6N7). ROBO1 shows multiple isoform variants. The variant used here is termed ROBO1b/DUTT1 (Genbank # NP_598334), and it possesses an 18 aa substitution for aa 1‑47, accompanied by a three aa insertion after Gln348, and a deletion of aa 939-947. ROBO1a, by contrast, possesses only the 18 aa substitution just described. A third isoform possesses the same changes as ROBO1b plus an additional deletion of aa 1013-1067, while a final variant utilizes an alternative start site at Met120. Proteolytic cleavage generates a soluble 120 kDa N-terminal fragment. Over aa 20-861, human ROBO1b shares 97% aa identity with mouse ROBO1.

Long Name
Roundabout Homolog 1
Entrez Gene IDs
6091 (Human); 58946 (Rat)
Alternate Names
DUTT1; DUTT1Drosophila) homolog 1; H-Robo-1; MGC131599; MGC133277; ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila)

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Citations for Human ROBO1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cdk5 and GSK3 beta inhibit fast endophilin-mediated endocytosis
    Authors: Antonio P. A. Ferreira, Alessandra Casamento, Sara Carrillo Roas, Els F. Halff, James Panambalana, Shaan Subramaniam et al.
    Nature Communications
  2. Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils
    Authors: D Pilling, LE Chinea, KM Consalvo, RH Gomer
    J. Immunol., 2018-12-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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