Home / S100A1 / Human S100A1 Antibody

Human S100A1 Antibody

Catalog #: AF4476
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4476
AF4476-SP
Detection of Human S100A1 by Western Blot.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S100A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) S100B, rhS100P, and rhS100A2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human S100A1
Met1-Ser94
Accession # P23297
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human S100A1 antibody by Western Blot. View Larger

Detection of Human S100A1 by Western Blot. Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4476) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A1

S100A1 (also S100 alpha) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by striated muscle, endothelium and chondrocytes. Intracellularly, S100A1 regulates cytosolic Ca levels, and is part of the Hsp70/Hsp90 mutlichaperone complex that protects against protein denaturation. Extracellularly, it exists as both a homodimer and heterodimer with S100B, S100A4 and S100P, may bind to RAGE, and blocks apoptosis. Human S100A1 is 94 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 50‑85) and a Ca-dependent protein interaction site (aa 89‑91). There may be one alternate start site 53 aa upstream of the standard start site. Full-length human S100A1 shares 93% and 98% aa identity with mouse and canine S100A1, respectively.

Long Name
S100 Calcium Binding Protein A1
Entrez Gene IDs
6271 (Human); 20193 (Mouse); 295214 (Rat)
Alternate Names
protein S100-A1; S100 alpha; S100 calcium binding protein A1; S100 calcium-binding protein A1S100; S-100 protein alpha chain; S-100 protein subunit alpha; S100 protein, alpha polypeptide; S100A; S100A1; S100-alpha

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human S100A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Circulating Ligands of the Receptor for Advanced Glycation End Products and the Soluble Form of the Receptor Modulate Cardiovascular Cell Apoptosis in Diabetes
    Authors: JN Tsoporis, E Hatziagela, S Gupta, S Izhar, V Salpeas, A Tsiavou, AG Rigopoulos, AS Triantafyl, JC Marshall, TG Parker, IK Rizos
    Molecules, 2020-11-10;25(22):.
    Species: Human
    Sample Types: Plasma
  2. S100A1 is released from ischemic cardiomyocytes and signals myocardial damage via Toll-like receptor 4.
    Authors: Rohde D, Schon C, Boerries M, Didrihsone I, Ritterhoff J, Kubatzky K, Volkers M, Herzog N, Mahler M, Tsoporis J, Parker T, Linke B, Giannitsis E, Gao E, Peppel K, Katus H, Most P
    EMBO Mol Med, 2014-04-06;6(6):778-94.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  3. S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation.
    Authors: Yamaguchi F, Umeda Y, Shimamoto S
    J. Biol. Chem., 2012-03-07;287(17):13787-98.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human S100A1 Antibody

There are currently no reviews for this product. Be the first to review Human S100A1 Antibody and earn rewards!

Have you used Human S100A1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review