Home / S100A8 / Human S100A8 DuoSet ELISA

Human S100A8 DuoSet ELISA

Catalog #: DY4570-05
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY4570-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human S100A8 ELISA Standard Curve
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human S100A8 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human S100A8. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human S100A8 ELISA Standard Curve View Larger

Human S100A8 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100A8

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name:
S100 Calcium Binding Protein A8
Entrez Gene IDs:
6279 (Human); 20201 (Mouse)
Alternate Names:
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A

Citations for Human S100A8 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Identification and characterization of plasma proteins associated with intra-amniotic inflammation and/or infection in women with preterm labor.
    Authors: Cho, HY;Lee, JE;Park, KH;Choi, BY;Lee, MJ;Jeong, DE;Shin, S;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  2. Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses
    Authors: Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS;
    Biomedicines
    Species: Human
    Sample Types: Whole Blood
  3. Inflammatory biomarkers in the cervicovaginal fluid to identify histologic chorioamnionitis and funisitis in women with preterm labor
    Authors: Park, KH;Lee, KN;Oh, E;Im, EM;
    Cytokine
    Species: Human
    Sample Types: Cervicovaginal Fluid
  4. Inflammation of Dry Eye Syndrome: A Cellular Study of the Epithelial and Macrophagic Involvement of NFAT5 and RAGE
    Authors: Henrioux, F;Navel, V;Belville, C;Charnay, C;Antoine, A;Chiambaretta, F;Sapin, V;Blanchon, L;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Using tears as a non-invasive source for early detection of breast cancer
    Authors: A Daily, P Ravishanka, S Harms, VS Klimberg
    PLoS ONE, 2022-04-26;17(4):e0267676.
    Species: Human
    Sample Types: Tears
  6. Characterization of low-density granulocytes in COVID-19
    Authors: LE Cabrera, PT Pekkarinen, M Alander, KHA Nowlan, NA Nguyen, S Jokiranta, S Kuivanen, A Patjas, S Mero, SH Pakkanen, S Heinonen, A Kantele, O Vapalahti, E Kekäläinen, T Strandin
    PloS Pathogens, 2021-07-06;17(7):e1009721.
    Species: Human
    Sample Types: Serum
  7. Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias
    Authors: M Raundhal, S Ghosh, SA Myers, MS Cuoco, M Singer, SA Carr, SS Waikar, JV Bonventre, J Ritz, RM Stone, DP Steensma, A Regev, LH Glimcher
    Nature Immunology, 2021-03-22;0(0):.
    Species: Human
    Sample Types: Plasma
  8. Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation
    Authors: H Xiang, F Guo, X Tao, Q Zhou, S Xia, D Deng, L Li, D Shang
    Theranostics, 2021-03-04;11(9):4467-4482.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Hypoxia-Induced S100A8 Expression Activates Microglial Inflammation and Promotes Neuronal Apoptosis
    Authors: JS Ha, HR Choi, IS Kim, EA Kim, SW Cho, SJ Yang
    International Journal of Molecular Sciences, 2021-01-26;22(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Heterogeneous bone-marrow stromal progenitors drive myelofibrosis via a druggable alarmin axis
    Authors: NB Leimkühler, HFE Gleitz, L Ronghui, IAM Snoeren, SNR Fuchs, JS Nagai, B Banjanin, KH Lam, T Vogl, C Kuppe, USA Stalmann, G Büsche, H Kreipe, I Gütgemann, P Krebs, Y Banz, P Boor, EW Tai, TH Brümmendor, S Koschmiede, M Crysandt, E Bindels, R Kramann, IG Costa, RK Schneider
    Cell Stem Cell, 2020-12-09;0(0):.
    Species: Human
    Sample Types: Plasma
  11. Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells
    Authors: J Ho, DN Wickramasi, SA Nikou, B Hube, JP Richardson, JR Naglik
    Cells, 2020-03-12;9(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads
    Authors: MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan
    PLoS ONE, 2018-01-25;13(1):e0191676.
    Species: Human
    Sample Types: Complex Sample Type
  13. A transcriptome-based global map of signaling pathways in the ovarian cancer microenvironment associated with clinical outcome
    Genome Biol, 2016-05-23;17(1):108.
    Species: Human
    Sample Types: Ascites Fluid

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