Human Serum Amyloid A1/A2 Antibody

Catalog #: MAB30192 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30192-100
MAB30192-SP

Detection of Human Serum Amyloid A1/A2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serum Amyloid A1/A2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SAA1/SAA2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 902728

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human SAA1 synthetic peptide
Accession # P0DJI8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Serum Amyloid A1/A2 by Western Blot. Western blot shows lysates of human spleen tissue, human serum, and human plasma. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Recombinant Human Serum Amyloid A1/A2 by Western Blot. Western blot shows 5 ng of Recombinant Human SAA1 and Recombinant Human SAA2. PVDF Membrane was probed with 0.2 µg/mL of Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30192) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A1/A2

Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL₃ complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.

Alternate Names

PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4; Serum Amyloid A1/A2