Human Serum Amyloid A1 Antibody

Catalog #: MAB3019
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3019
MAB3019-SP

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serum Amyloid A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serum Amyloid A1 (SAA1) in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant mouse SAA1 and no cross-reactivity with recombinant human (rh) SAA4 is observed. In direct ELISAs, no cross-reactivity with rhSAA2 is observed.

Source

Monoclonal Rat IgG₁ Clone # 370820

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SAA1
Arg19-Tyr122
Accession # P02735

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Serum Amyloid A1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A1

Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL₃ complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.

Entrez Gene IDs

6288 (Human); 20208 (Mouse)

Alternate Names

MGC111216; PIG4; SAA; SAA1; SAA2; serum amyloid A protein; Serum Amyloid A1; TP53I4; tumor protein p53 inducible protein 4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Serum Amyloid A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1