Home / Siglec-11 / Human Siglec-11 Antibody

Human Siglec-11 Antibody

Catalog #: MAB3258
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3258
MAB3258-SP
Detection of Siglec‑11 in HEK293 Human Cell Line Transfected with Human Siglec-11 and eGFP by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with Siglec-16 is observed.
Source
Monoclonal Mouse IgG2A Clone # 705904
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Siglec-11 synthetic peptide
PPARLSWTRWGQTVGPSQPSDPGVLELPPIC
Accession # Q96RL6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec-11 antibody in HEK293 Human Cell Line Transfected with Human Siglec-11 and eGFP antibody by Flow Cytometry. View Larger

Detection of Siglec‑11 in HEK293 Human Cell Line Transfected with Human Siglec-11 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-11 or (B) human Siglec-16 and eGFP was stained with Mouse Anti-Human Siglec-11 Monoclonal Antibody (Catalog # MAB3258) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and. Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry Siglec-11 antibody in Human Lymph Node by Immunohistochemistry (IHC-P). View Larger

Siglec‑11 in Human Lymph Node. Siglec-11 was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human Siglec-11 Monoclonal Antibody (Catalog # MAB3258) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-11

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-set domain which mediates sialic acid binding, followed by a varying numbers of Ig-like C2-set domains. Siglecs‑3 and 5‑13 constitute the CD33/Siglec-3 related group, which are defined by their sequence homology and differential expression in the hematopoietic system (1‑3). Mature human Siglec-11 consists of a 534 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain. The ECD contains one Ig-like V-set domain, and three Ig-like C2-set domains. The cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (4). A splice variant of Siglec-11 has a deletion of nearly 100 aa in the extracellular juxtamembrane region. Among siglecs, the ECD of Siglec-11 is most closely related to that of Siglec-10 (82% aa sequence identity). The cytoplasmic domains of these proteins are only 20% identical. Siglec-11 is closely related to the pseudogenes Siglec-14 and Siglec-16 (4, 5). Human Siglec-11 shares 90%‑96% aa sequence identity with Siglec-11 from great apes. Rodent orthologs of Siglec-11 have not been identified. In human, Siglec-11 is expressed in tissue macrophages, brain microglia, and inflammatory site monocytes (4). Strong microglial expression is specific to humans, as it is less prominent or absent in chimpanzees and orangutans (5). Siglec-11 forms 180 kDa disulfide-linked dimers. It shows a strong binding preference for sialic acid in alpha 2-8 linkage which is unusual for siglecs (4). A conserved arginine in the Ig-like V-set domain only partially contributes to Siglec-11 ligand recognition, in contrast to its being required in other siglecs (4). Tyrosine phosphorylation of the cytoplasmic region of Siglec-11 promotes association with the phosphatases SHP-1 and SHP-2 (4).

References
  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
  3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Hayakawa, T. et al. (2005) Science 309:1693.
Long Name
Sialic Acid Binding Ig-like Lectin 11
Entrez Gene IDs
114132 (Human)
Alternate Names
sialic acid binding Ig-like lectin 11; sialic acid-binding Ig-like lectin 11; Sialic acid-binding lectin 11; Siglec11; Siglec-11

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Citations for Human Siglec-11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The Paired Siglecs in Brain Tumours Therapy: The Immunomodulatory Effect of Dexamethasone and Temozolomide in Human Glioma In Vitro Model
    Authors: P Wielgat, N Wawrusiewi, R Czarnomysy, K Rogowski, K Bielawski, H Car
    International Journal of Molecular Sciences, 2021-02-11;22(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Paired Siglec receptors generate opposite inflammatory responses to a human‐specific pathogen
    Authors: Flavio Schwarz, Corinna S Landig, Shoib Siddiqui, Ismael Secundino, Joshua Olson, Nissi Varki et al.
    The EMBO Journal

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