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Human Siglec-7/CD328 Antibody

Catalog #: AF1138
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1138
AF1138-SP
Cell Adhesion Mediated by Siglec‑7 and Neutralization by Human Siglec‑7 Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-7/CD328 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec‑7/CD328 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human (rh) Siglec-9 is observed and approximately 5% cross-reactivity with recombinant mouse Siglec-E, rhSiglec-6, and rhSiglec-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-7/CD328
Gln19-Gly357
Accession # Q9Y286
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Siglec‑7/CD328 Fc Chimera (Catalog # 1138-SL)
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human lung
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize Siglec‑7-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND50) is typically 0.3-0.9 µg/mL in the presence of 1.5 µg/mL Recombinant Human Siglec‑7 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Adhesion Mediated by Siglec‑7 and Neutralization by Human Siglec‑7 Antibody. View Larger

Cell Adhesion Mediated by Siglec‑7 and Neutralization by Human Siglec‑7 Antibody. Recombinant Human Siglec-7 Fc Chimera (Catalog # 1138-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-7 Fc Chimera (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Siglec-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1138). The ND50 is typically 0.3-0.9 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-7/CD328

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

Human Siglec-7 encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5). Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8+ T cells and monocytes (3, 5). It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid (5). The gene encoding Siglec-7 was mapped to chromosome 19q13.3.

References
  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Nicoll, G. et al. (1999) J. Biol. Chem. 274:34089.
Long Name
Sialic Acid Binding Ig-like Lectin 7
Entrez Gene IDs
27036 (Human)
Alternate Names
Adhesion inhibitory receptor molecule 1; adhesion inhibitory receptor molecule 1, siglec-7; AIRM1; AIRM-1; AIRM1QA79 membrane protein; CD328 antigen; CD328; CDw328; D-siglec; p75; p75/AIRM1; QA79; sialic acid binding Ig-like lectin 7; sialic acid binding immunoglobulin-like lectin 7; sialic acid-binding Ig-like lectin 7; Siglec7; Siglec-7

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Citations for Human Siglec-7/CD328 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7
    Authors: M Vijayan, CL Lee, VHH Wong, X Wang, K Bai, J Wu, H Koistinen, M Seppälä, KF Lee, WSB Yeung, EHY Ng, PCN Chiu
    J. Cell. Sci., 2020-07-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Siglec-7 restores ?-cell function and survival and reduces inflammation in pancreatic islets from patients with diabetes
    Authors: G Dharmadhik, K Stolz, M Hauke, NG Morgan, A Varki, E de Koning, S Kelm, K Maedler
    Sci Rep, 2017-04-05;7(0):45319.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  3. Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
    Authors: Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J
    PLoS Biol, 2012-12-18;10(12):e1001448.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Engagement of Siglec-7 receptor induces a pro-inflammatory response selectively in monocytes.
    Authors: Varchetta S, Brunetta E, Roberto A, Mikulak J, Hudspeth K, Mondelli M, Mavilio D
    PLoS ONE, 2012-09-28;7(9):e45821.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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