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Human Siglec-9 Antibody

Catalog #: MAB1139
7 Citations Datasheet / COA / SDS
Clone 191240 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1139-SP
MAB1139-500
MAB1139-100
Detection of Siglec‑9 in Human Blood Granulocytes by Flow Cytometry.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec-9 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse
Siglec‑E is observed and less than 1% cross-reactivity with recombinant human (rh) Siglec-3, rhSiglec-5, rhSiglec-6, rhSiglec-7, rhSiglec-8 or rhSiglec-10 is observed. In Western blots, approximately 100% cross-reactivity with recombinant mouse Siglec‑E is observed under
non-reduced conditions.
Source
Monoclonal Mouse IgG2A Clone # 191240
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-9
Gln18-Gly348 (predicted)
Accession # Q9Y336
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Siglec‑9 Fc Chimera (Catalog # 1139-SL) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human Siglec-9 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Siglec‑9 Biotinylated Antibody (Catalog # BAF1139)

Standard: Recombinant Human Siglec-9 Fc Chimera Protein, CF (Catalog # 1139-SL)

Neutralization
Measured by its ability to neutralize Siglec‑9-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND50) is typically 0.015-0.075 µg/mL in the presence of 10 µg/mL Recombinant Human Siglec‑9 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec-9 antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of Siglec‑9 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and (B) Mouse Anti-Human Siglec-9 Monoclonal Antibody (Catalog # MAB1139, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0114).

Neutralization Cell Adhesion Mediated by Siglec‑9 and Neutralization by Human Siglec‑9 Antibody. View Larger

Cell Adhesion Mediated by Siglec‑9 and Neutralization by Human Siglec‑9 Antibody. Recombinant Human Siglec-9 Fc Chimera (Catalog # 1139-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-9 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-9 Monoclonal Antibody (Catalog # MAB1139). The ND50 is typically 0.015-0.075 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-9

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

References
  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
  6. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.
Long Name
Sialic Acid Binding Ig-like Lectin 9
Entrez Gene IDs
27180 (Human); 102118310 (Cynomolgus Monkey)
Alternate Names
CD329; CDw329; FOAP-9; OBBP-Like; Protein FOAP-9; sialic acid binding Ig-like lectin 9; sialic acid-binding Ig-like lectin 9; Siglec9; Siglec-9

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Citations for Human Siglec-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Wogonin attenuates neutrophilic inflammation and airway smooth muscle proliferation through inducing caspase-dependent apoptosis and inhibiting MAPK/Akt signaling in allergic airways
    Authors: F Bai, Z Chen, S Xu, L Han, X Zeng, S Huang, Z Zhu, L Zhou
    International immunopharmacology, 2022-11-11;113(0):109410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7
    Authors: M Vijayan, CL Lee, VHH Wong, X Wang, K Bai, J Wu, H Koistinen, M Seppälä, KF Lee, WSB Yeung, EHY Ng, PCN Chiu
    J. Cell. Sci., 2020-07-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment
    Authors: Q Haas, K Frias Boli, C Jandus, C Schneider, C Simillion, MA Stanczak, M Haubitz, SM Seyed Jafa, A Zippelius, GM Baerlocher, H Läubli, RE Hunger, P Romero, HU Simon, S von Gunten
    Cancer Immunol Res, 2019-04-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Self-associated molecular patterns mediate cancer immune evasion by engaging Siglecs on T cells
    Authors: MA Stanczak, SS Siddiqui, MP Trefny, DS Thommen, KF Boligan, S von Gunten, A Tzankov, L Tietze, D Lardinois, V Heinzelman, MS von Bergwe, W Zhang, HJ Lenz, Y Han, CI Amos, M Syedbasha, A Egli, F Stenner, DE Speiser, A Varki, A Zippelius, H Läubli
    J. Clin. Invest., 2018-09-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010-08-06;5(8):e11966.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Molecular mimicry of host sialylated glycans allows a bacterial pathogen to engage neutrophil Siglec-9 and dampen the innate immune response.
    Authors: Carlin AF, Uchiyama S, Chang YC, Lewis AL, Nizet V, Varki A
    Blood, 2009-02-04;113(14):3333-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Tamm–Horsfall glycoprotein engages human Siglec‐9 to modulate neutrophil activation in the urinary tract
    Authors: Kathryn A. Patras, Alison Coady, Joshua Olson, Syed Raza Ali, Satish P. RamachandraRao, Satish Kumar et al.
    Immunology & Cell Biology

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