Human SIRP beta 2 Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: SIRP beta 2

Signal-regulatory protein beta-2(SIRP-beta-2), is a ~37 kDa monomeric single pass type I membrane glycoprotein. It belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). SIRP-beta-2 contains an N-terminal signal peptide (aa1-32), two extracellular Ig-like domains: a V-type 1 (aa 33-143) and a V-type 2 (aa 157-258) containing three potential N-linked glycosylation sites, a helical transmembrane domain (aa 288-308), and a cytoplasmic domain (aa 309-342) (1). A positively charged residue within the transmembrane domain, in analogy to SIRP-beta-1, is implicated to mediate interaction with the adaptor DAP12 protein, which contains immunoreceptor tyrosine-based activation motifs (ITAMs) (2). Proteins in the SIRP family are typically expressed in immune cells, especially in the myeloid lineages (3). Based on expression patterns, SIRPs are thought to have roles in immune regulation (4). SIRP family members role in innate immunity and host defense has potential significance as a therapeutic target in cancer and inflammation (5, 6). There are currently no known mouse or rat homologs for this protein.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• Dendritic Cell ITIM/ITAM Immunoreceptors
• Epigenetics
• ITIM/ITAM Immunoreceptors and Related Molecules
• Phosphatases and Regulators
• Search Kinases and Phosphatases