Human SIRP beta 2 Antibody

Catalog #: MAB99981 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB99981-100
MAB99981-SP

Detection of SIRP beta 2 in HEK293 Human Cell Line Transfected with Human SIRP beta 2 and eGFP by Flow Cytometry.

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All SIRP beta 2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SIRP beta 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SIRP beta 2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1008702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line, HEK293 derived Human SIRP beta 2
Gln33-Gly287
Accession # Q5JXA9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SIRP beta 2 in HEK293 Human Cell Line Transfected with Human SIRP beta 2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) SIRP beta 2 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human SIRP beta 2 Monoclonal Antibody (Catalog # MAB99981) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of SIRP beta 2 in THP-1 Cells by Flow Cytometry. THP-1 monocytic leukemia cell line was stained with Mouse Anti-Human SIRP beta 2 Monoclonal Antibody (Catalog # MAB99981, filled histogram) or Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP beta 2

Signal-regulatory protein beta-2(SIRP-beta-2), is a ~37 kDa monomeric single pass type I membrane glycoprotein. It belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). SIRP-beta-2 contains an N-terminal signal peptide (aa1-32), two extracellular Ig-like domains: a V-type 1 (aa 33-143) and a V-type 2 (aa 157-258) containing three potential N-linked glycosylation sites, a helical transmembrane domain (aa 288-308), and a cytoplasmic domain (aa 309-342) (1). A positively charged residue within the transmembrane domain, in analogy to SIRP-beta-1, is implicated to mediate interaction with the adaptor DAP12 protein, which contains immunoreceptor tyrosine-based activation motifs (ITAMs) (2). Proteins in the SIRP family are typically expressed in immune cells, especially in the myeloid lineages (3). Based on expression patterns, SIRPs are thought to have roles in immune regulation (4). SIRP family members role in innate immunity and host defense has potential significance as a therapeutic target in cancer and inflammation (5, 6). There are currently no known mouse or rat homologs for this protein.

References

van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
Liu, Y. et al. (2005) Journal of Biological Chemistry. 280:36132.
Matozaki, T. et al. (2009) Trends in Cell Biology. 19:72.
Barclay A.N. et al. (2006) Nat Rev Immunol. 6:457.
Barclay A.N. et al. (2014) Annu Rev Immunol. 32:25.
Veillette A. (2018) Trends Immunol. 39:173.

Long Name

Signal-regulatory Protein beta 2

Entrez Gene IDs

284759 (Human)

Alternate Names

dJ776F14.2; Protein Tyrosine Phosphatase Non-Receptor Type Substrate 1-Like 3; Protein Tyrosine Phosphatase Non-Receptor Type Substrate Protein; Protein Tyrosine Phosphatase, Non-Receptor Type 1-Like; Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1-Like 3; PTPN1L; PTPNS1L3; Signal-Regulatory Protein Beta 2; Signal-Regulatory Protein Beta-2; SIRP beta 2; SIRP-beta-2; SIRPG