Human SLC5A5 Antibody

Catalog # Availability Size / Price Qty
MAB8367
MAB8367-SP
Detection of SLC5A5 in HEK293 Human Cell Line Transfected with Human SLC5A5 and eGFP by Flow Cytometry.
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Human SLC5A5 Antibody Summary

Species Reactivity
Human
Specificity
Stains human SLC5A5 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 902320
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human SLC5A5
Accession # Q92911
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SLC5A5 antibody in HEK293 Human Cell Line Transfected with Human SLC5A5 and eGFP antibody by Flow Cytometry. View Larger

Detection of SLC5A5 in HEK293 Human Cell Line Transfected with Human SLC5A5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human SLC5A5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human SLC5A5 Monoclonal Antibody (Catalog # MAB8367) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC5A5

Solute Carrier, family 5 (sodium iodide symporter), member 5 (SLC5A5) is a multi-pass symporter for sodiom/iodide uptake.  SLC5A5 is mainly expressed in thyroid follicular cells, and it promotes tumor cell invasiveness.

Long Name
Solute Carrier Family 5 (Sodium Iodide Symporter), Member 5
Entrez Gene IDs
6528 (Human); 114479 (Mouse); 114613 (Rat)
Alternate Names
Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; NIS; NISNa(+)/I(-)-symporter; SLC5A5; sodium/iodide cotransporter; Sodium-iodide symporter; solute carrier family 5 (sodium iodide symporter), member 5; Solute carrier family 5 member 5; TDH1

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