Human SPARC-like 1/SPARCL1 Antibody Summary
Ile17-Phe664
Accession # Q8N4S1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SPARC‑like 1/SPARCL1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SPARC-like 1/ SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC-like 1/SPARCL1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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SPARC‑like 1/SPARCL1 in Human Brain. SPARC-like 1/SPARCL1 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of SPARC‑like 1/SPARCL1 in HL‑60 Human Cell Line by Flow Cytometry. HL-60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPARC-like 1/SPARCL1
SPARCL1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins (1, 2). SPARCL1 is an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver (3, 4). Human SPARCL1 contains a 16 amino acid (aa) signal sequence and a 648 aa mature region with four domains: a 416 aa N-terminal acidic region, a 23 aa follistatin-like domain, a 55 aa kazal-like segment and a 48 aa EF-hand/calcium-binding domain (3, 4). SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfide-linked homodimerization or by glycosylation and high acidity (3-5). Some truncated forms have been reported. In mouse, a 55 kDa C-terminal fragment is the only form in kidney and represents a portion of SPARCL1 in other tissues (6). In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the full-length form is downregulated in many epithelial cell-derived tumors (7, 8). SPARCL1 inhibits adhesion and spreading on a variety of substrates (5, 9). It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma (10). In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation (3). SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin (6, 11). Human mature SPARCL1 shares 67%, 69%, 78%, 76%, 72%, and 72% aa identity with mouse, rat, equine, canine, porcine and bovine SPARCL1, respectively. The follistatin-like, kazal-like and calcium-binding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC.
- Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
- Sullivan, M.M. and E.H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991.
- Girard, J.P. and T.A. Springer (1995) Immunity 2:113.
- Bendik, I. et al. (1998) Cancer Res. 58:626.
- Brekken, R.A. et al. (2004) J. Histochem. Cytochem. 52:735.
- Hambrock, H.O. et al. (2003) J. Biol. Chem. 278:11351.
- Lau, C.P. et al. (2006) J. Pathol. 210:469.
- Isler, S.G. et al. (2001) Int. J. Oncol. 18:521.
- Girard, J.P. and T.A. Springer (1996) J. Biol. Chem. 271:4511.
- Gongidi, V. et al. (2004) Neuron 41:57.
- Sullivan, M.M. et al. (2006) J. Biol. Chem. 281:27621.
Product Datasheets
Citations for Human SPARC-like 1/SPARCL1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Matricellular Protein SPARCL1 Regulates Blood Vessel Integrity and Antagonizes Inflammatory Bowel Disease
Authors: Daniela Regensburger, Clara Tenkerian, Victoria Pürzer, Benjamin Schmid, Thomas Wohlfahrt, Iris Stolzer et al.
Inflammatory Bowel Diseases
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Clinicopathological Significance of Reduced SPARCL1 Expression in Human Breast Cancer
Authors: Fang Cao, Kuo Wang, Rong Zhu, Yong-Wei Hu, Wen-Zheng Fang, Hou-Zhong Ding
Asian Pacific Journal of Cancer Prevention
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Molecular and cellular dynamics of the developing human neocortex at single-cell resolution
Authors: Wang, L;Wang, C;Moriano, JA;Chen, S;Zuo, G;Cebrián-Silla, A;Zhang, S;Mukhtar, T;Wang, S;Song, M;de Oliveira, LG;Bi, Q;Augustin, JJ;Ge, X;Paredes, MF;Huang, EJ;Alvarez-Buylla, A;Duan, X;Li, J;Kriegstein, AR;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Immunohistochemistry, Immunocytochemistry -
Tandem Mass Tag LC-MS/MS of Aqueous Humor From Individuals With Type 2 Diabetes Without Retinopathy Reveals Early Dysregulation of Synaptic Proteins
Authors: Sachdeva, MM;Lee, Y;Unlu, EK;Koseoglu, ND;Cha, E;Wang, J;Prescott, CR;Eghrari, AO;Na, CH;
Investigative ophthalmology & visual science
Species: Human
Sample Types: Aqueous Humor
Applications: Western Blot -
Heterogeneity of glial progenitor cells during the neurogenesis-to-gliogenesis switch in the developing human cerebral cortex
Authors: Y Fu, M Yang, H Yu, Y Wang, X Wu, J Yong, Y Mao, Y Cui, X Fan, L Wen, J Qiao, F Tang
Cell Reports, 2021-03-02;34(9):108788.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Species-, organ- and cell-type-dependent expression of SPARCL1 in human and mouse tissues
Authors: A Klingler, D Regensburg, C Tenkerian, N Britzen-La, A Hartmann, M Stürzl, E Naschberge
PLoS ONE, 2020-05-21;15(5):e0233422.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
SPARCL1, a Novel Prognostic Predictive Factor for GI Malignancies: a Meta-Analysis
Authors: H Hu, W Cai, S Zheng, W Ge
Cell. Physiol. Biochem., 2017-12-01;44(4):1485-1496.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Molecular resistance fingerprint of pemetrexed and platinum in a long-term survivor of mesothelioma.
Authors: Roe O, Szulkin A, Anderssen E, Flatberg A, Sandeck H, Amundsen T, Erlandsen S, Dobra K, Sundstrom S
PLoS ONE, 2012-08-08;7(8):e40521.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Down-regulated SPARCL1 is associated with clinical significance in human gastric cancer.
Authors: Li P, Qian J, Yu G, Chen Y, Liu K, Li J, Wang J
J Surg Oncol, 2011-08-30;105(1):31-7.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Sparc-Like Protein 1 Is a New Marker of Human Glioma Progression
Authors: Andrei Turtoi, Davide Musmeci, Antonio Giuseppe Naccarato, Cristian Scatena, Valerio Ortenzi, Robert Kiss et al.
Journal of Proteome Research
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Matricellular protein SPARCL1 regulates tumor microenvironment–dependent endothelial cell heterogeneity in colorectal carcinoma
Authors: Elisabeth Naschberger, Andrea Liebl, Vera S. Schellerer, Manuela Schütz, Nathalie Britzen-Laurent, Patrick Kölbel et al.
Journal of Clinical Investigation
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