Human STEAP1 Antibody

Catalog #: MAB55871 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB55871-100
MAB55871-SP

Detection of STEAP1 in HEK293 cells by Flow Cytometry.

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All STEAP1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human STEAP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human STEAP1 in direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2959F

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human STEAP1
Met1-Trp71
Accession # Q9UHE8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

Human prostate cancer and LNCaP

Simple Western

10 µg/mL

LNCaP human prostate cancer cells

Flow Cytometry

0.25 µg/10⁶ cells

see below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of STEAP1 in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human STEAP-1 and eGFP (A) vs irrelevant and eGFP (B) were stained with Rabbit Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB55871) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Western Blot

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Detection of Human STEAP1 by Western Blot. Western blot shows lysates of Human prostate cancer and LNCaP. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB55871) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STEAP1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human STEAP1 by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cells, loaded at 0.2 mg/mL. A specific band was detected for STEAP1 at approximately 39 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB55871). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Flow Cytometry

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Detection of STEAP1 in LnCaP cells by Flow Cytometry LnCaP cells were stained with Rabbit Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB55871, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STEAP1

STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40 kDa protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell‑cell junctions between prostate secretory epithelium, and up‑regulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339 amino acids (aa) in length. It contains a ferric oxidoreductase domain (aa 119‑265) that includes transmembrane sequences (# 2, 3, 4, and part of 5, out of 6 sequences). Two splice forms diverge at aa 255, terminating after aa 58 or 259. Over aa 1‑71, human and mouse STEAP1 share 68% aa identity.

Long Name

Six Transmembrane Epithelial Antigen of the Prostate 1

Entrez Gene IDs

26872 (Human); 70358 (Mouse); 297738 (Rat)

Alternate Names

EC 1.16.1.-; PRSS24; PRSS24MGC19484; six transmembrane epithelial antigen of the prostate 1; six transmembrane epithelial antigen of the prostate; Six-transmembrane epithelial antigen of prostate 1; STEAP1; STEAPmetalloreductase STEAP1