Human STRO-1 Alexa Fluor® 488-conjugated Antibody

Discontinued Product

FAB1038G has been discontinued.
View all STRO-1 products.
Detection of STRO-1 in MG-63 Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Human STRO-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human STRO-1.
Source
Monoclonal Mouse IgM Clone # STRO-1
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Human CD34+ bone marrow cells
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of STRO-1 antibody in MG-63 Cell Line antibody by Flow Cytometry. View Larger

Detection of STRO-1 in MG-63 Cell Line by Flow Cytometry. Human MG-63 osteosarcoma cell line was stained with Mouse Anti-Human STRO-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1038G, filled histogram) or Mouse IgM isotype control antibody (Catalog # IC015G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitution default not known
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STRO-1

STRO-1 is a cell surface protein expressed by bone marrow stromal cells and erythroid precursors. The frequency of colony forming units-fibroblasts (CFU-F) was enriched 100-fold in the STRO-1+/Glycophorin A- population from bone marrow cells (1). The subset of marrow cells that expresses the STRO-1 antigen is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts, and chondrocytes (2).

References
  1. Simmons, P.J. and B. Torok-Storb (1991) Blood 78:55.
  2. Dennis, J.E. et al. (2002) Cells Tissues Organs 170:73.
Long Name
A Stromal Cell Precursor Surface Antigen
Alternate Names
STRO1; STRO-1

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human STRO-1 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells
    Authors: Xiaoyu Li, Bowen Zhang, Hong Wang, Xiaolu Zhao, Zijie Zhang, Gang Ding et al.
    J. Int. Med. Res
  2. Aspirin promotes osteogenic differentiation of human dental pulp stem cells
    Authors: Mengtong Yuan, Yuanbo Zhan, Weiping Hu, Ying Li, Xiaohua Xie, Nan Miao et al.
    International Journal of Molecular Medicine

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