Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Survivin Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
Sensitivity
9.96 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human Survivin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Survivin immunoassay is a 4.5 hour solid phase ELISA designed to measure human Survivin in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human Survivin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Survivin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Survivin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 137 | 398 | 841 | 164 | 445 | 879 |
| Standard Deviation | 6.41 | 27.3 | 44.2 | 14.6 | 39.4 | 63.8 |
| CV% | 4.7 | 6.9 | 5.3 | 8.9 | 8.9 | 7.3 |
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 190 | 513 | 982 | 197 | 529 | 1060 |
| Standard Deviation | 7.78 | 17.4 | 53.8 | 18.8 | 37.3 | 59.9 |
| CV% | 4.1 | 3.4 | 5.5 | 9.5 | 7.1 | 5.6 |
Recovery
The recovery of Survivin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 88-109 |
| EDTA Plasma (n=4) | 96 | 89-109 |
| Heparin Plasma (n=4) | 96 | 87-110 |
| Serum (n=4) | 93 | 85-109 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Survivin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Survivin
Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.
Entrez Gene IDs:
332 (Human)
Alternate Names:
API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Survivin Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
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Survivin is a negative prognostic factor in malignant pleural effusion
Authors: E Arellano-O, B Romero-Rom, V Sánchez-Ló, J Martín-Jua, F Rodríguez-, R Otero-Cand
Eur. J. Clin. Invest., 2018-02-13;0(0):.
Species: Human
Sample Types: Pleural Fluid
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Racial differences in the expression of inhibitors of apoptosis (IAP) proteins in extracellular vesicles (EV) from prostate cancer patients
Authors: S Khan, J Simpson, JC Lynch, D Turay, S Mirshahidi, A Gonda, TW Sanchez, CA Casiano, NR Wall
PLoS ONE, 2017-10-05;12(10):e0183122.
Species: Human
Sample Types: Plasma
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Evaluation of tissue metalloproteinase inhibitor TIMP-1 and Survivin levels during third trimester pregnancy - a preliminary report
Authors: A Karowicz-B, U Kowalska-K, D Estemberg, A Sikora-Szu
Ginekol. Pol., 2017-01-01;88(4):198-204.
Species: Human
Sample Types: Serum
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Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients
Oncol Lett, 2016-09-01;12(5):3591-3597.
Species: Human
Sample Types: Serum
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Serum survivin increases in prolactinoma.
Authors: Dellal, Fatma Di, Niyazoglu, Mutlu, Gorar, Suheyla, Ademoglu, Esranur, Candan, Zehra, Bekdemir, Handan, Hacioglu, Yalcin, Kaya, Fatih On
J Clin Med Res, 2015-02-09;7(4):248-52.
Species: Human
Sample Types: Serum
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Prognostic significance of pretreatment VEGF, survivin, and Smac/DIABLO serum levels in patients with serous ovarian carcinoma.
Authors: Dobrzycka B, Mackowiak-Matejczyk B, Terlikowska K, Kulesza-Bronczyk B, Kinalski M, Terlikowski S
Tumour Biol, 2015-01-12;0(0):4157.
Species: Human
Sample Types: Serum
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Clinical significance of serum survivin in patients with pancreatic ductal adenocarcinoma.
Authors: Ren, Y-Q, Zhang, H-Y, Su, T, Wang, X-H, Zhang, L
Eur Rev Med Pharmacol Sci, 2014-10-01;18(20):3063-8.
Species: Human
Sample Types: Serum
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Early diagnostic value of survivin and its alternative splice variants in breast cancer.
Authors: Khan S, Bennit H, Turay D, Perez M, Mirshahidi S, Yuan Y, Wall N
BMC Cancer, 2014-03-12;14(0):176.
Species: Human
Sample Types: Serum
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Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer.
Authors: Khan, Salma, Jutzy, Jessica, Valenzuela, Malyn Ma, Turay, David, Aspe, Jonathan, Ashok, Arjun, Mirshahidi, Saied, Mercola, Dan, Lilly, Michael, Wall, Nathan R
PLoS ONE, 2012-10-16;7(10):e46737.
Species: Human
Sample Types: Plasma
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Extracellular, cell-permeable survivin inhibits apoptosis while promoting proliferative and metastatic potential.
Authors: Khan S, Aspe JR, Asumen MG, Almaguel F, Odumosu O, Acevedo-Martinez S, De Leon M, Langridge WH, Wall NR
Br. J. Cancer, 2009-03-17;100(7):1073-86.
Species: Human
Sample Types: Cell Culture Supernates
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Reviews for Human Survivin Quantikine ELISA Kit
Average Rating: 4 (Based on 4 Reviews)
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Human Survivin Quantikine ELISA Kit
By Anonymous
on 03/28/2022
Sample Tested: Cell culture supernatant
Human Survivin Quantikine ELISA Kit
By Anonymous
on 01/03/2022
Sample Tested: Serum
R^2 is great, but the absorbance values are not. Most of the samples were below the zero.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
Human Survivin Quantikine ELISA Kit
By Anonymous
on 07/27/2021
Sample Tested: Cell Culture Media,EDTA Plasma
Human Survivin Quantikine ELISA Kit
By Adam Guess
on 08/17/2018
Sample Tested: Cell Culture Supernates
