Human Survivin Quantikine ELISA Kit Summary
Product Summary
Recovery
The recovery of Survivin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 88-109 |
| EDTA Plasma (n=4) | 96 | 89-109 |
| Heparin Plasma (n=4) | 96 | 87-110 |
| Serum (n=4) | 93 | 85-109 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Survivin
Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Survivin Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
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Survivin is a negative prognostic factor in malignant pleural effusion
Authors: E Arellano-O, B Romero-Rom, V Sánchez-Ló, J Martín-Jua, F Rodríguez-, R Otero-Cand
Eur. J. Clin. Invest., 2018-02-13;0(0):.
Species: Human
Sample Types: Pleural Fluid
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Racial differences in the expression of inhibitors of apoptosis (IAP) proteins in extracellular vesicles (EV) from prostate cancer patients
Authors: S Khan, J Simpson, JC Lynch, D Turay, S Mirshahidi, A Gonda, TW Sanchez, CA Casiano, NR Wall
PLoS ONE, 2017-10-05;12(10):e0183122.
Species: Human
Sample Types: Plasma
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Evaluation of tissue metalloproteinase inhibitor TIMP-1 and Survivin levels during third trimester pregnancy - a preliminary report
Authors: A Karowicz-B, U Kowalska-K, D Estemberg, A Sikora-Szu
Ginekol. Pol., 2017-01-01;88(4):198-204.
Species: Human
Sample Types: Serum
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Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients
Oncol Lett, 2016-09-01;12(5):3591-3597.
Species: Human
Sample Types: Serum
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Serum survivin increases in prolactinoma.
Authors: Dellal, Fatma Di, Niyazoglu, Mutlu, Gorar, Suheyla, Ademoglu, Esranur, Candan, Zehra, Bekdemir, Handan, Hacioglu, Yalcin, Kaya, Fatih On
J Clin Med Res, 2015-02-09;7(4):248-52.
Species: Human
Sample Types: Serum
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Prognostic significance of pretreatment VEGF, survivin, and Smac/DIABLO serum levels in patients with serous ovarian carcinoma.
Authors: Dobrzycka B, Mackowiak-Matejczyk B, Terlikowska K, Kulesza-Bronczyk B, Kinalski M, Terlikowski S
Tumour Biol, 2015-01-12;0(0):4157.
Species: Human
Sample Types: Serum
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Clinical significance of serum survivin in patients with pancreatic ductal adenocarcinoma.
Authors: Ren, Y-Q, Zhang, H-Y, Su, T, Wang, X-H, Zhang, L
Eur Rev Med Pharmacol Sci, 2014-10-01;18(20):3063-8.
Species: Human
Sample Types: Serum
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Early diagnostic value of survivin and its alternative splice variants in breast cancer.
Authors: Khan S, Bennit H, Turay D, Perez M, Mirshahidi S, Yuan Y, Wall N
BMC Cancer, 2014-03-12;14(0):176.
Species: Human
Sample Types: Serum
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Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer.
Authors: Khan, Salma, Jutzy, Jessica, Valenzuela, Malyn Ma, Turay, David, Aspe, Jonathan, Ashok, Arjun, Mirshahidi, Saied, Mercola, Dan, Lilly, Michael, Wall, Nathan R
PLoS ONE, 2012-10-16;7(10):e46737.
Species: Human
Sample Types: Plasma
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Extracellular, cell-permeable survivin inhibits apoptosis while promoting proliferative and metastatic potential.
Authors: Khan S, Aspe JR, Asumen MG, Almaguel F, Odumosu O, Acevedo-Martinez S, De Leon M, Langridge WH, Wall NR
Br. J. Cancer, 2009-03-17;100(7):1073-86.
Species: Human
Sample Types: Cell Culture Supernates
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R^2 is great, but the absorbance values are not. Most of the samples were below the zero.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
