Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 918842
View all Synaptopodin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Synaptopodin
Ser363-Gln490
Accession # Q8N3V7

Specificity

Detects human Synaptopodin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Synaptopodin Antibody

Synaptopodin antibody in Human Kidney by Immunohistochemistry (IHC-P).

Synaptopodin in Human Kidney.

Synaptopodin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Synaptopodin Monoclonal Antibody (Catalog # MAB8977) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence

Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence

Double immunofluorescence staining of NLRC5 and glomerular intrinsic cells in AAV patients. a Co-localization of NLRC5 (red) and CD31 (green). b Co-localization of NLRC5 (red) and integrin-alpha (green). c Co-localization of NLRC5 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186034), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence

Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence

Double immunofluorescence staining of NLRP3 and glomerular intrinsic cells in AAV patients. a Co-localization of NLRP3 (red) and CD31 (green). b Co-localization of NLRP3 (red) and integrin-alpha (green). c Co-localization of NLRP3 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186034), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence

Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence

Double immunofluorescence staining of NOD2 and glomerular intrinsic cells in AAV patients. a Co-localization of NOD2 (red) and CD31 (green). b Co-localization of NOD2 (red) and integrin-alpha (green). c Co-localization of NOD2 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186034), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Synaptopodin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Synaptopodin

Synaptopodin is a 929 amino acid, actin associated protein with restricted expression in brain and kidney.  First reported in glomerular podocytes, the protein is also  found in postsynaptic densities (PSDs) in a subpopulation of telencephalic neurons where it is thought to participate in synaptic plasticity in dendritic spines.  Despite its localization in PSDs, it is not homologous to other PSD proteins.  Loss of synaptopodin expression has been demonstrated in Alzheimer’s patients. In glomeruli, synaptopodin localizes to the cytoplasm of podocyte foot processes. 

Alternate Names

SYNPO

Entrez Gene IDs

11346 (Human); 104027 (Mouse); 60324 (Rat)

Gene Symbol

SYNPO

UniProt

Q8N3V7

Additional Synaptopodin Products

Product Documents for Human Synaptopodin Antibody

Certificate of Analysis

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Product Specific Notices for Human Synaptopodin Antibody

For research use only

Citations for Human Synaptopodin Antibody

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Protocols

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