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Human Synaptopodin Antibody

Catalog #: MAB8977
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8977
MAB8977-SP
Synaptopodin in Human Kidney.
4 Images
Product Details
Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Synaptopodin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Synaptopodin in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 918842
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Synaptopodin
Ser363-Gln490
Accession # Q8N3V7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Synaptopodin antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Synaptopodin in Human Kidney. Synaptopodin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Synaptopodin Monoclonal Antibody (Catalog # MAB8977) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence Double immunofluorescence staining of NLRC5 and glomerular intrinsic cells in AAV patients. a Co-localization of NLRC5 (red) and CD31 (green). b Co-localization of NLRC5 (red) and integrin-alpha (green). c Co-localization of NLRC5 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186034), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence Double immunofluorescence staining of NLRP3 and glomerular intrinsic cells in AAV patients. a Co-localization of NLRP3 (red) and CD31 (green). b Co-localization of NLRP3 (red) and integrin-alpha (green). c Co-localization of NLRP3 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186034), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence Double immunofluorescence staining of NOD2 and glomerular intrinsic cells in AAV patients. a Co-localization of NOD2 (red) and CD31 (green). b Co-localization of NOD2 (red) and integrin-alpha (green). c Co-localization of NOD2 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186034), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Synaptopodin

Synaptopodin is a 929 amino acid, actin associated protein with restricted expression in brain and kidney.  First reported in glomerular podocytes, the protein is also  found in postsynaptic densities (PSDs) in a subpopulation of telencephalic neurons where it is thought to participate in synaptic plasticity in dendritic spines.  Despite its localization in PSDs, it is not homologous to other PSD proteins.  Loss of synaptopodin expression has been demonstrated in Alzheimer’s patients. In glomeruli, synaptopodin localizes to the cytoplasm of podocyte foot processes. 

Entrez Gene IDs
11346 (Human); 104027 (Mouse); 60324 (Rat)
Alternate Names
KIAA1029; Synaptopodin; SYNPO

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Citations for Human Synaptopodin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. KIBRA upregulation increases susceptibility to glomerular injury and correlates with kidney function decline
    Authors: Meliambro K, Yang Y, de Cos M et al.
    JCI insight
  2. Deficiency of CFB attenuates renal tubulointerstitial damage by inhibiting ceramide synthesis in diabetic kidney disease
    Authors: ZJ Sun, DY Chang, M Chen, MH Zhao
    JCI Insight, 2022-12-22;7(24):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Renal Expression of Annexin A1 Is Associated With the Severity of Renal Injury in Antineutrophil Cytoplasmic Autoantibody-Associated Vasculitis
    Authors: Rui-Xue Wang, Liang Wu, Su-Fang Chen, Zhi-Ying Li, Ming-Hui Zhao, Min Chen
    Frontiers in Medicine
  4. The expression of NOD2, NLRP3 and NLRC5 and renal injury in anti-neutrophil cytoplasmic antibody-associated vasculitis
    Authors: LY Wang, XJ Sun, M Chen, MH Zhao
    J Transl Med, 2019-06-11;17(1):197.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  5. Tyro3 is a podocyte protective factor in glomerular disease
    Authors: F Zhong, Z Chen, L Zhang, Y Xie, V Nair, W Ju, M Kretzler, RG Nelson, Z Li, H Chen, Y Wang, A Zhang, K Lee, Z Liu, JC He
    JCI Insight, 2018-11-15;3(22):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Blockage of the lysosome-dependent autophagic pathway contributes to complement membrane attack complex-induced podocyte injury in idiopathic membranous nephropathy
    Authors: WJ Liu, ZH Li, XC Chen, XL Zhao, Z Zhong, C Yang, HL Wu, N An, WY Li, HF Liu
    Sci Rep, 2017-08-17;7(1):8643.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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