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Human Syndecan-1/CD138 Antibody

Catalog #: AF2780
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2780
AF2780-SP
Syndecan‑1/CD138 in Human Ileum.
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Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Syndecan-1/CD138 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4, and recombinant mouse Syndecan-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
Gln18-Glu251
Accession # NP_002988
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD)
Immunohistochemistry
5-15 µg/mL
See below

Human Syndecan-1/CD138 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human Syndecan‑1/CD138 Biotinylated Antibody (Catalog # BAF2780)

Standard: Recombinant Human Syndecan-1 Protein, CF (Catalog # 2780-SD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Syndecan‑1/CD138 antibody in Human Ileum by Immunohistochemistry (IHC-P). View Larger

Syndecan‑1/CD138 in Human Ileum. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human ileum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Syndecan-1/CD138 antibody in Human Jejunum by Immunohistochemistry (IHC-P). View Larger

Syndecan‑1/CD138 in Human Jejunum. Syndecan-1/CD138 was detected in immersion fixed paraffin-embedded sections of human jejunum using Human Syndecan-1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-1/CD138

Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.

References
    Entrez Gene IDs
    6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)
    Alternate Names
    CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1

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    Citations for Human Syndecan-1/CD138 Antibody

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    13 Citations: Showing 1 - 10
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    1. Cellular and inter-cellular rewiring of the human colon during ulcerative colitis
      Authors: Christopher S. Smillie, Moshe Biton, Jose Ordovas-Montanes, Keri M. Sullivan, Grace Burgin, Daniel B. Graham et al.
      Cell
    2. Estrogen receptor alpha regulates the expression of syndecan-1 in human breast carcinoma cells
      Authors: Emmanuelle Fleurot, Caroline Goudin, Vincent Hanoux, Pierre-Jacques Bonnamy, Jérôme Levallet
      Endocrine-Related Cancer
    3. The extracellular matrix of lymph node reticular fibers modulates follicle border interactions and germinal center formation
      Authors: Jian Song, Tushar Deshpande, Xueli Zhang, Melanie-Jane Hannocks, Nils Lycke, Susanna L. Cardell et al.
      iScience
    4. NGF-Enhanced Vasculogenic Properties of Epithelial Ovarian Cancer Cells Is Reduced by Inhibition of the COX-2/PGE2 Signaling Axis
      Authors: MP Garrido, I Hurtado, M Valenzuela, R Salvatierr, A Hernández, M Vega, A Selman, AFG Quest, C Romero
      Cancers (Basel), 2019-12-07;11(12):.
      Species: Human
      Sample Types: Tissue Lysates
      Applications: Western Blot
    5. Augmented concentrations of CX3CL1 are associated with interstitial lung disease in systemic sclerosis
      Authors: AM Hoffmann-V, SS Weigt, V Palchevski, E Volkmann, R Saggar, N Li, Ø Midtvedt, MB Lund, T Garen, MC Fishbein, A Ardehali, DJ Ross, T Ueland, P Aukrust, JP Lynch, RM Elashoff, Ø Molberg, JA Belperio
      PLoS ONE, 2018-11-20;13(11):e0206545.
      Species: Human
      Sample Types: Whole Tissue
      Applications: IHC-P
    6. GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation
      Authors: S Offenbache, Y Jiao, SJ Kim, J Marchesan, KL Moss, L Jing, K Divaris, S Bencharit, CS Agler, T Morelli, S Zhang, L Sun, WT Seaman, D Cowley, SP Barros, JD Beck, M Munz, AS Schaefer, KE North
      Nat Commun, 2018-09-11;9(1):3686.
      Species: Human
      Sample Types: Whole Tissue
      Applications: IHC
    7. Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device
      Authors: MA Qasaimeh, YC Wu, S Bose, A Menachery, S Talluri, G Gonzalez, M Fulciniti, JM Karp, RH Prabhala, R Karnik
      Sci Rep, 2017-04-04;7(0):45681.
      Species: Human
      Sample Types: Whole Cells
      Applications: Immunoprecipitation
    8. Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.
      Authors: Purushothaman A, Bandari S, Liu J, Mobley J, Brown E, Sanderson R
      J Biol Chem, 2015-11-24;291(4):1652-63.
      Species: Human
      Sample Types: Whole Cells
      Applications: Flow Cytometry
    9. Shed syndecan-1 translocates to the nucleus of cells delivering growth factors and inhibiting histone acetylation: a novel mechanism of tumor-host cross-talk.
      Authors: Stewart M, Ramani V, Sanderson R
      J Biol Chem, 2014-11-17;290(2):941-9.
      Species: Human, Mouse
      Sample Types: Cell Lysates
      Applications: Western Blot
    10. Chemotherapy stimulates syndecan-1 shedding: a potentially negative effect of treatment that may promote tumor relapse.
      Authors: Ramani V, Sanderson R
      Matrix Biol, 2013-10-18;35(0):215-22.
      Species: Human
      Sample Types: Cell Lysates, Whole Cells
      Applications: Immunodepletion, Western Blot
    11. Novel processed form of syndecan-1 shed from SCC-9 cells plays a role in cell migration.
      Authors: Aragao A, Belloni M, Simabuco F, Zanetti M, Yokoo S, Domingues R, Kawahara R, Pauletti B, Goncalves A, Agostini M, Graner E, Coletta R, Fox J, Leme A
      PLoS ONE, 2012-08-15;7(8):e43521.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    12. Heparanase Plays a Dual Role in Driving Hepatocyte Growth Factor (HGF) Signaling by Enhancing HGF Expression and Activity.
      Authors: Ramani VC, Yang Y, Ren Y
      J. Biol. Chem., 2010-12-03;286(8):6490-9.
      Species: Human
      Sample Types: Cell Culture Supernates
      Applications: Immunodepletion
    13. CD28-mediated regulation of multiple myeloma cell proliferation and survival.
      Authors: Bahlis NJ, King AM, Kolonias D, Carlson LM, Liu HY, Hussein MA, Terebelo HR, Byrne GE, Levine BL, Boise LH, Lee KP
      Blood, 2007-02-20;109(11):5002-10.
      Species: Human
      Sample Types: Whole Tissue
      Applications: IHC

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