Human TBX18 Antibody

Catalog #: MAB6337
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6337
MAB6337-SP

Detection of Human and Mouse TBX18 by Western Blot.

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All TBX18 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TBX18 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TBX18 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 635303

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TBX18
Ala384-Ser504
Accession # O95935

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse TBX18 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB6337) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TBX18 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX18

TBX18 is a 607 amino acid, 65 kDa (predicted) protein that is a member of the T-box family of developmentally expressed transcription factors. It is developmentally expressed in the anterior portion of the head, somites and limb buds. It is expressed on pluripotent progenitors in the developing epicardium where it is required for formation of the cardiac AV node, and can also contribute to fibroblast and smooth muscle formation. Within the region used as an immunogen, human TBX18 shares 93% amino acid identity with mouse and rat TBX18.

Long Name

T-box Transcription Factor 18

Entrez Gene IDs

9096 (Human); 76365 (Mouse); 315870 (Rat)

Alternate Names

T-box 18; T-box protein 18; T-box transcription factor TBX18; TBX18

Product Datasheets

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Citation for Human TBX18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1