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Human TCF-3/E2A Antibody

Catalog #: MAB44061
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB44061
MAB44061-SP
TCF-3/E2A in HeLa Human Cell Line.
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Human TCF-3/E2A Antibody Summary

Species Reactivity
Human
Specificity
Detects human TCF-3/E2A in direct ELISAs. In this format, 100% cross-reactivity with recombinant human (rh) TCF-12 is observed and no cross-reactivity with rhTCF-1, 2, 4, 7L1, 7L2 or rhLEF-1 is observed.
Source
Monoclonal Rat IgG2B Clone # 401709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TCF-3/E2A
Gly100-Glu500
Accession # NP_003191
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TCF-3/E2A antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

TCF-3/E2A in HeLa Human Cell Line. TCF-3/E2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-3/E2A

TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively.

Long Name
Transcription Factor 3/E2-alpha
Entrez Gene IDs
6929 (Human); 21423 (Mouse); 312451 (Rat)
Alternate Names
bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; TCF-3; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor

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Citation for Human TCF-3/E2A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MicroRNA-449a maintains self-renewal in liver cancer stem-like cells by targeting Tcf3
    Authors: Q Zhang, Z Yang, J Shan, L Liu, C Liu, J Shen, X Chen, Y Xu, J Chen, Q Ma, L Yang, C Qian
    Oncotarget, 2017-11-27;8(66):110187-110200.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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