Human TCL1A Antibody

Catalog #: AF4847 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4847
AF4847-SP

TCL1A in Daudi Human Burkitt's Lymphoma Cells.

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Human TCL1A Antibody Summary

Species Reactivity

Human

Specificity

Detects human TCL1A in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TCL1A
Met1-Asp114
Accession # P56279

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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TCL1A in Daudi Human Burkitt's Lymphoma Cells. TCL1A was detected in immersion fixed Daudi human Burkitt's lymphoma cell line using 10 µg/mL Goat Anti-Human TCL1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4847) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot

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Detection of Human TCL1A by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TCL1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4847) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human TCL1A was included. A specific band for TCL1A was detected at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCL1A

TCL1A (T cell leukemia protein 1A; also p14 TCL1) is a 14 kDa member of the TCL1 family of proteins. It is expressed by thymocytes and activated T and B lymphocytes, and interacts with Akt kinase, promoting its activation and the subsequent deactivation of proapoptotic molecules. Human TCL1A is 114 amino acids (aa) in length. The amino acids Asp16 and Ile74 mediate Akt association, while Pro36 Leu37 Thr38 mediates TCL1A homodimerization. Full length human TCL1A is 50% aa identical to mouse TCL1A.

Long Name

T Cell Leukemia Protein 1A

Entrez Gene IDs

8115 (Human); 21432 (Mouse); 690575 (Rat)

Alternate Names

Oncogene TCL1; Oncogene TCL-1; Protein p14 TCL1; T-cell leukemia/lymphoma 1A; T-cell lymphoma-1; T-cell lymphoma-1A; TCL1A; TCL1-PEN; TCL1T-cell leukemia/lymphoma protein 1A