Human TEM7/PLXDC1 Isoform 1 Antibody

Catalog #: AF5327 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5327
AF5327-SP

Detection of Human TEM7/PLXDC1 Isoform 1 by Western Blot.

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Human TEM7/PLXDC1 Isoform 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TEM7/PLXDC1 Isoform 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TEM8 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TEM7/PLXDC1 Isoform 1
Leu19-Thr426
Accession # ACE86780

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TEM7/PLXDC1 Isoform 1 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line cancer tissue, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TEM7/PLXDC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5327) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEM7 Isoform 1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TEM7/PLXDC1

TEM7 (Tumor endothelial marker 7; also TEM3 and Plexin domain-containing protein-1/PXDC1) is an 85 kDa member of the plexin family of proteins. It is one of a number of TEM designated molecules that are related only a name and not structural similarity. TEM7 is found on tumor endothelial cells, newly formed endothelial cells and neurons. It is known to bind nidogen. Mature human TEM7 is a type I transmembrane glycoprotein 482 amino acids (aa) in length. It contains an extracellular region (aa 19‑426) with one plexin domain (aa 303‑347). There are three isoform variants. One is intracellular and shows an alternate start site at Met74 accompanied by a 28 aa substitution for aa 331‑500. The other two are soluble, with one showing only the above 28 aa substitution and a second showing an eight aa substitution for aa 331‑500. Over aa 19‑426, human TEM7 shares 81% aa identity with mouse TEM7.

Long Name

Tumor Endothelial Marker 7/Plexin Domain Containing Protein 1

Entrez Gene IDs

57125 (Human); 72324 (Mouse); 303505 (Rat)

Alternate Names

FLJ45632; plexin domain containing 1,2410003I07Rik; plexin domain-containing protein 1; PLXDC1; TEM3; TEM3FLJ36270; TEM7; TEM7DKFZp686F0937; Tumor endothelial marker 3; Tumor endothelial marker 7