Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Teneurin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Teneurin‑1
Met1-Lys317
Accession # AAF04723

Specificity

Detects human Teneurin-1 in direct ELISAs and Western blots.  In direct ELISAs, approximately 15% cross-reactivity with recombinant human Teneurin-3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Teneurin‑1 Antibody

Detection of Human Teneurin-1 antibody by Western Blot.

Detection of Human Teneurin‑1 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human Teneurin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Teneurin-1 by Western Blot

Detection of Teneurin-1 by Western Blot

RNA interference by p38-specific siRNAs neutralizes the EGF-promoted upregulation of ODZ1. (A) GBM cells were transfected with two MAPK14-specific siRNAs or two control siRNAs. Following incubation with EGF, the protein levels of ODZ1 were determined by Western Blot analysis. (B) Same experimental design as in A but uses MAPK11-specific siRNAs. Additionally, alpha Tubulin was used to ensure equal loading. (C) Quantification of ODZ1 protein levels of the experiment shown in B by using imageJ software (v1.53k). (D) Cells carrying siRNAs specific to MAPK11 were analyzed for the expression of either MAPK11 or MAPK14 mRNA by qPCR. Histograms represent the mean of three independent experiments ± S.D. ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Teneurin-1 by Western Blot

Detection of Teneurin-1 by Western Blot

The blockade of the EGFR–p38 signaling downregulates the levels of ODZ1. (A) GBM cells were treated with EGF in the absence or in the presence of the EGFR-blocking antibody Cetuximab or the specific p38 MAPK inhibitor SB203580. Western Blot analysis confirmed that the blockade of EGFR/p38 inhibited the phosphorylation of MAPKAPK2 at Thr222, a target of p38, and reduced the expression of the ODZ1 protein. (B) Downregulation of ODZ1 following inhibition of EGFR/p38 was confirmed in two additional GBM cell lines. MAPKAPK2 and alpha Tubulin were used to ensure equal loading. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Teneurin-1 by Western Blot

Detection of Teneurin-1 by Western Blot

The blockade of the EGFR–p38 signaling downregulates the levels of ODZ1. (A) GBM cells were treated with EGF in the absence or in the presence of the EGFR-blocking antibody Cetuximab or the specific p38 MAPK inhibitor SB203580. Western Blot analysis confirmed that the blockade of EGFR/p38 inhibited the phosphorylation of MAPKAPK2 at Thr222, a target of p38, and reduced the expression of the ODZ1 protein. (B) Downregulation of ODZ1 following inhibition of EGFR/p38 was confirmed in two additional GBM cell lines. MAPKAPK2 and alpha Tubulin were used to ensure equal loading. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Teneurin-1 by Western Blot

Detection of Teneurin-1 by Western Blot

RNA interference by p38-specific siRNAs neutralizes the EGF-promoted upregulation of ODZ1. (A) GBM cells were transfected with two MAPK14-specific siRNAs or two control siRNAs. Following incubation with EGF, the protein levels of ODZ1 were determined by Western Blot analysis. (B) Same experimental design as in A but uses MAPK11-specific siRNAs. Additionally, alpha Tubulin was used to ensure equal loading. (C) Quantification of ODZ1 protein levels of the experiment shown in B by using imageJ software (v1.53k). (D) Cells carrying siRNAs specific to MAPK11 were analyzed for the expression of either MAPK11 or MAPK14 mRNA by qPCR. Histograms represent the mean of three independent experiments ± S.D. ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Teneurin-1 by Western Blot

Detection of Teneurin-1 by Western Blot

EGF induces the expression of ODZ1. (A) FACS analysis of two GBM cell lines expressing, or not, EGFR (same as in Figure 2A). (B) GBM cells were incubated with EGF and the mRNA levels of ODZ1 were determined after 24 h by qPCR. Histograms represent the mean of three independent experiments ± S.D. Asterisks represent significant differences (** p < 0.01). (C) Western Blot analysis confirmed that the exposure of GBM cells to EGF also promoted the expression of ODZ1 at the protein level. Additionally, alpha Tubulin was used to ensure equal loading. Arrows indicate the native high-molecular-weight ODZ1 protein and its most frequent proteolytic fragment of 70 kDa. (D) Cells were incubated in the presence of EGF and migration was determined at different time points by using an in vitro wound healing assay. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38727302), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Teneurin‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Teneurin-1

Teneurin-1 (also Ten-m1, tenascin-M1, and Ten-m/Odz1) is a 250-300 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar granule cells and CA2 pyramidal hippocampal neurons. Teneurin 1 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions. Human Teneurin-1 is a 2725 amino acid (aa) type II TM glycoprotein. It contains a 324 aa cytoplasmic region (aa 1-324) that contains an NLS (aa 62‑65), plus a 2380 aa extracellular domain (ECD). The ECD possesses eight sequential EGF-like domains (aa 528-796), five NHL repeats, each of which form a  beta -propeller (aa 1194-1524), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. Cleavage at the N-terminus generates an initial 65 kDa membrane spanning fragment, followed by TM cleavage that generates a 45 kDa cytosolic fragment. C-terminal cleavage generates a short 5 kDa, 41 aa peptide (aa 2682-2722) termed TCAP-1 that shows bioactivity. One splice variant shows a deletion of aa 1232-1239. Over aa 1-317, human Teneurin-1 shares 96% aa identity with mouse Teneurin-1.

Alternate Names

ODZ1, ODZ3, Ten-1, TEN-M1, Tenascin-M1, Teneurin1, TNM

Entrez Gene IDs

10178 (Human); 23963 (Mouse)

Gene Symbol

TENM1

UniProt

AAF04723

Additional Teneurin-1 Products

Product Documents for Human Teneurin‑1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Teneurin‑1 Antibody

For research use only

Citations for Human Teneurin‑1 Antibody

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