Human Teneurin-1 Antibody Summary
Met1-Lys317
Accession # AAF04723
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Teneurin‑1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human Teneurin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Teneurin-1
Teneurin-1 (also Ten-m1, tenascin-M1, and Ten-m/Odz1) is a 250-300 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar granule cells and CA2 pyramidal hippocampal neurons. Teneurin 1 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions. Human Teneurin-1 is a 2725 amino acid (aa) type II TM glycoprotein. It contains a 324 aa cytoplasmic region (aa 1-324) that contains an NLS (aa 62‑65), plus a 2380 aa extracellular domain (ECD). The ECD possesses eight sequential EGF-like domains (aa 528-796), five NHL repeats, each of which form a beta -propeller (aa 1194-1524), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. Cleavage at the N-terminus generates an initial 65 kDa membrane spanning fragment, followed by TM cleavage that generates a 45 kDa cytosolic fragment. C-terminal cleavage generates a short 5 kDa, 41 aa peptide (aa 2682-2722) termed TCAP-1 that shows bioactivity. One splice variant shows a deletion of aa 1232-1239. Over aa 1-317, human Teneurin-1 shares 96% aa identity with mouse Teneurin-1.
Product Datasheets
Citations for Human Teneurin-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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The Invasion Factor ODZ1 Is Upregulated through an Epidermal Growth Factor Receptor-Induced Pathway in Primary Glioblastoma Cells
Authors: Velasquez, C;Gutierrez, O;Carcelen, M;Fernandez-Luna, JL;
Cells
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ODZ1 allows glioblastoma to sustain invasiveness through a Myc-dependent transcriptional upregulation of RhoA
Oncogene, 2016-09-19;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot
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