Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 353816
View all TfR2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TfR2
Arg105-Phe801
Accession # Q9UP52

Specificity

Detects human TfR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TfR1 or recombinant mouse TfR2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TfR2 Antibody

Detection of Human TfR2 antibody by Western Blot.

Detection of Human TfR2 by Western Blot.

Western blot shows lysates of human liver tissue and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TfR2 Monoclonal Antibody (Catalog # MAB3120) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TfR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human TfR2 antibody by Simple WesternTM.

Detection of Human TfR2 by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for TfR2 at approximately 104 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human TfR2 Monoclonal Antibody (Catalog # MAB3120). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human TfR2 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human liver tissue

Western Blot

2 µg/mL
Sample: Human liver tissue and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TfR2

Transferrin Receptor 2 (TfR2) is an iron-binding protein that is homologous to the transferrin receptor. Lack of active TfR2 has been associated with a rare form of hemochromatosis. The TfR2 alpha isoform is a type 2 transmembrane protein that is expressed in the liver and modulates hepcidin production in response to iron. It forms a 215 kDa disulfide-linked homodimer that is presumably glycated. The TfR2 beta isoform lacks the intracellular, transmembrane and part of the extracellular regions and is expressed ubiquitously. The extracellular portion of human TfR2 shares 86% aa identity with mouse.

Long Name

Transferrin Receptor 2

Alternate Names

HFE3, TFRC2

Entrez Gene IDs

7036 (Human); 50765 (Mouse)

Gene Symbol

TFR2

UniProt

Q9UP52

Additional TfR2 Products

Product Documents for Human TfR2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TfR2 Antibody

For research use only

Related Research Areas

Acute Phase Proteins

Citations for Human TfR2 Antibody

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