Human Thymidine Kinase 1 Antibody

Catalog #: MAB81801 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB81801-100
MAB81801-SP

Detection of Human Thymidine Kinase 1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Thymidine Kinase 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Thymidine Kinase 1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2627A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived human Thymidine Kinase 1
Ser2-Asn234
Accession # P04183

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

HeLa human cervical epithelial carcinoma cell line

Simple Western

10 µg/mL

A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line and SK‑BR‑3 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Thymidine Kinase 1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Thymidine Kinase 1 Monoclonal Antibody (Catalog # MAB81801) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Thymidine Kinase 1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human Thymidine Kinase 1 by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line and SK‑BR‑3 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Thymidine Kinase 1 at approximately 32 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Thymidine Kinase 1 Monoclonal Antibody (Catalog # MAB81801). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thymidine Kinase 1

Thymidine kinases are 2'-deoxythymidine kinases that phosphorylate deoxythymidine and generate deoxythymidine 5’-phosphate (1). Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Two forms of thymidine kinase are present in mammalian cells, TK1 and TK2. TK1 is cell cycle-dependent and is present in the cytoplasm only in anticipation of cell division (2, 3); whereas TK2 is located in mitochondria and is cell cycle-independent (4). TK1 is synthesized by the cell during the S phase of cell division and is degraded after cell division is completed (5). TK1 normally occurs in tissue as a dimer and can be converted to more active tetrameric form by ATP binding (6). TK1 is feedback inhibited by thymidine triphosphate, the product of the further phosphorylation of thymidine (7). Because tumor cells replicate much more frequently than normal cells and requires faster DNA synthesis and higher TK1 activity, TK1 is a cancer maker especially for hematologic malignancies (8, 9). In clinical chemistry TK1 is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease (10). In addition, thymidine kinase is required for the action of many antiviral drugs (11). The enzymatic activity of recombinant human TK1 is measured using a phosphatase-coupled method (12).

References

Wintersberger, E. (1997) Biochem. Soc. Trans. 25:303.
Littelfield, J.W. (1966) Biochim. Biophys. Acta 114:398.
Flemington, E, et al. (1987) Gene 52:267.
Berk, A.J. et al. (1973) Arch. Biochem. Biophys. 154:563.
Zhu, C. et al. (2006) Nucleosides Nucleotides Nucleic Acids 25:1185.
Munch-Petersen, B. et al. (1993) J. Biol. Chem. 268:15621.
Mikkelsen, N. E. et al. (2003). Biochemistry 42:5706.
Doi S, et al. (1990) Nagoya J. Med. Sci. 52:19.
Ellims PH, et al. (1981). Cancer Res. 41: 691.
Lin TS, et al. (1976) J. Med. Chem.19:495.
Baba M, et al. (1987). Biochem. Biophys. Res. Commun. 142:128.
Wu, Z.L. (2011) PLoS One 6:e23172.

Entrez Gene IDs

7083 (Human); 21877 (Mouse)

Alternate Names

EC 2.7.1.21; thymidine kinase 1 soluble isoform; Thymidine Kinase 1; thymidine kinase 1, soluble; thymidine kinase, cytosolic; thymidine kinase-1; TK1; TK2