Human Thyroglobulin Antibody Summary
Met1-Lys2768
Accession # P01266
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Thyroglobulin by Western Blot. Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Thyroglobulin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Thyroglobulin in Human Thyroid Cancer Tissue. Thyroglobulin was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thyroglobulin
Human Thyroglobulin (Tg) gene is localized at position 8q242-8q243. The gene covers a genome area of at least 300 000 bp and contains at least 37 exons. Thyroglobulin (Tg) is a dimeric 660 kDa glycoprotein produced by follicular cells (thyrocytes) in thyroid gland and stored in the follicular colloid of the gland. Tg is heterogenic due to post-translational modifications, such as iodination, glycosylation, sulfatation, phosphorylation and it is highly sensitivity to proteolysis. Acting as a substrate, Tg is used for the synthesis of thyroid hormones thyroxine (T4) and triiodothyronine (T3). Tg level can serve as a tumor marker of papillary and follicular thyroid cancer and elevated levels of anti-Tg antibodies in blood can be indicative of Graves disease.
Product Datasheets
FAQs
-
What antigen retrieval method was used to generate the IHC staining of human thyroid cancer tissue with Anti-human Thyroglobulin Monoclonal Antibody, Catalog # MAB8306?
Heat Induced Epitope Retreival (HIER) method was used in house with basic antigen retrieval (pH 9).
Reviews for Human Thyroglobulin Antibody
There are currently no reviews for this product. Be the first to review Human Thyroglobulin Antibody and earn rewards!
Have you used Human Thyroglobulin Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
