Human TIMP-1 Antibody

Name: Human TIMP-1 Antibody
Brand: R&D Systems
SKU: MAB970
Rating: 5 (4 reviews)

Catalog #: MAB970
19 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB970-SP
MAB970-500
MAB970-100

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All TIMP-1 Antibodies

Product Details

Citations (19)

FAQs

Supplemental Products

Reviews (4)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIMP-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TIMP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse TIMP‑1 or recombinant human TIMP‑2.

Source

Monoclonal Mouse IgG_2B Clone # 63515

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # P01033

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TIMP-1 Western Blot Standard (Catalog # WBC021)
under non-reducing conditions only

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

3-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Human T cells fixed with paraformaldehyde and permeabilized with saponin

Human TIMP-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human TIMP‑1 Biotinylated Antibody (Catalog # BAF970)

Standard: Recombinant Human TIMP-1 Protein, CF (Catalog # 970-TM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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TIMP‑1 in Wi-38 Human Cell Line. TIMP-1 was detected in immersion fixed Wi-38 human lung fibroblast cell line using Mouse Anti-Human TIMP-1 Monoclonal Antibody (Catalog # MAB970) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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TIMP‑1 in Human Astrocytoma. TIMP-1 was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Mouse Anti-Human TIMP-1 Monoclonal Antibody (Catalog # MAB970) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Alternate Names

CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TIMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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High levels of tissue inhibitor of metalloproteinase-1 (TIMP-1) in the serum are associated with poor prognosis in HPV-negative squamous cell oropharyngeal cancer
Authors: Timo Carpén, Timo Sorsa, Lauri Jouhi, Taina Tervahartiala, Caj Haglund, Stina Syrjänen et al.
Cancer Immunology, Immunotherapy
Effect of Pirfenidone on TGF-beta1-Induced Myofibroblast Differentiation and Extracellular Matrix Homeostasis of Human Orbital Fibroblasts in Graves' Ophthalmopathy
Authors: SB Wu, TY Hou, HC Kau, CC Tsai
Biomolecules, 2021-09-29;11(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
JNK and p38 Inhibitors Prevent Transforming Growth Factor-&beta1-Induced Myofibroblast Transdifferentiation in Human Graves' Orbital Fibroblasts
Authors: TY Hou, SB Wu, HC Kau, CC Tsai
International Journal of Molecular Sciences, 2021-03-14;22(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
TIMP-1-Mediated Chemoresistance via Induction of IL-6 in NSCLC
Authors: W Xiao, L Wang, J Howard, R Kolhe, AM Rojiani, MV Rojiani
Cancers (Basel), 2019-08-15;11(8):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Tissue Inhibitor of Metalloproteinase-1 Promotes Polymorphonuclear Neutrophil (PMN) Pericellular Proteolysis by Anchoring Matrix Metalloproteinase-8 and -9 to PMN Surfaces
Authors: X Wang, J Rojas-Quin, J Wilder, Y Tesfaigzi, D Zhang, CA Owen
J. Immunol., 2019-04-24;0(0):.
Species: Primate
Sample Types: Whole Cells
Applications: ICC
Increased expression of matrix metalloproteinase-1 in systemic vessels of preeclamptic women: a critical mediator of vascular dysfunction.
Authors: Estrada-Gutierrez G, Cappello RE, Mishra N, Romero R, Strauss JF, Walsh SW
Am. J. Pathol., 2010-12-23;178(1):451-60.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010-06-08;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Authors: Cavallo C, Desando G, Facchini A, Grigolo B
J Biomed Mater Res A, 2010-04-01;93(1):86-95.
Species: Human
Sample Types: Whole Cells
Applications: ICC
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
Biol. Reprod., 2010-01-14;82(5):968-79.
Species: Human
Sample Types: Whole Cells
Applications: Cell-based ELISA
Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
Authors: Lucio-Eterovic AK, Piao Y, de Groot JF
Clin. Cancer Res., 2009-06-30;15(14):4589-99.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ICC, Western Blot

Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Exp. Hematol., 2009-05-03;37(7):849-56.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Matrix metalloproteinase activity in pediatric acute lung injury.
Authors: Kong MY, Gaggar A, Li Y
Int J Med Sci, 2008-12-16;6(1):9-17.
Species: Human
Sample Types: Tissue Secretion
Applications: Western Blot

Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
Mol. Cell Proteomics, 2008-04-03;7(10):1974-82.
Species: Human
Sample Types: Serum
Applications: Mass Spectrometry

IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
J. Immunol., 2007-05-01;178(9):5957-65.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
Authors: Naveau A, Reinald N, Fournier B
Connect. Tissue Res., 2007-01-01;48(6):300-8.
Species: Human
Sample Types: Whole Cells
Applications: Dot Blot

Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut, 2006-05-08;55(12):1774-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

GPI-anchored TIMP-1 treatment renders renal cell carcinoma sensitive to FAS-meditated killing.
Authors: Djafarzadeh R, Noessner E, Engelmann H
Oncogene, 2006;25(10):1496-508.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Limited processing of pro-matrix metalloprotease-2 (gelatinase A) overexpressed by transfection in PC-3 human prostate tumor cells: association with restricted cell surface localization of membrane-type matrix metalloproteinase-1.
Authors: Wilson MJ, Jiang A, Wiehr C, Wang X, Sinha AA, Pei D
J. Androl., 2004-03-01;25(2):274-85.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

Serum Fucosylated Prostate-specific Antigen (PSA) Improves the Differentiation of Aggressive from Non-aggressive Prostate Cancers
Authors: Qing Kay Li, Li Chen, Ming-Hui Ao, Joyce Hanching Chiu, Zhen Zhang, Hui Zhang et al.
Theranostics