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Human TIMP-2 Antibody

Catalog #: MAB971
14 Citations 1 Review Datasheet / COA / SDS
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MAB971
MAB971-SP
Detection of Human TIMP‑2 by Western Blot.
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Product Details
Citations (14)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIMP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIMP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TIMP-1 or recombinant mouse TIMP-1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 89025
Purification
Protein A or G purified from ascites
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2
Cys27-Pro220
Accession # P16035
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TIMP-2 antibody by Western Blot. View Larger

Detection of Human TIMP‑2 by Western Blot. Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated).This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TIMP‑2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

TIMP‑2 in Human Ovarian Cancer Tissue. TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-2

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.

Long Name
Tissue Inhibitors of Metalloproteinases 2
Entrez Gene IDs
7077 (Human); 21858 (Mouse); 29543 (Rat)
Alternate Names
CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2

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Citations for Human TIMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. PAI-1 uncouples integrin-?1 from restrain by membrane-bound ?-catenin to promote collagen fibril remodeling in obesity-related neoplasms
    Authors: Lin, LL;Nayak, B;Osmulski, PA;Wang, E;Wang, CP;Valente, PT;Wang, CM;Tan, X;Santanam, N;Wang, TL;Gaczynska, ME;Kost, ER;Huang, TH;Kirma, NB;
    Cell reports
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Western Blot, Immunocytochemistry, Functional Assay
  2. PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling
    Authors: Backe, SJ;Votra, SD;Stokes, MP;Sebesty�n, E;Castelli, M;Torielli, L;Colombo, G;Woodford, MR;Mollapour, M;Bourboulia, D;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  3. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction
    Authors: Gerwinn T, Salemi S, Schori LJ et al.
    Frontiers in Cell and Developmental Biology
  4. Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/ beta -Catenin Pathway
    Authors: Yang Wang, Qi Yang, Yanli Cheng, Meng Gao, Lei Kuang, Chun Wang
    Medical Science Monitor
  5. MicroRNA?122 acts as tumor suppressor by targeting TRIM29 and blocking the activity of PI3K/AKT signaling in nasopharyngeal carcinoma in�vitro
    Authors: Y Yang, Q Li, L Guo
    Mol Med Rep, 2018-04-19;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors
    Authors: Angela Armento, Elena I. Ilina, Tony Kaoma, Arnaud Muller, Laurent Vallar, Simone P. Niclou et al.
    International Journal of Oncology
  7. Enhanced Immunoreactivity of TIMP-2 in the Stromal Compartment of Tumor as a Marker of Favorable Prognosis in Ovarian Cancer Patients
    Authors: Agnieszka Hałoń, Ewa Nowak-Markwitz, Piotr Donizy, Rafał Matkowski, Adam Maciejczyk, Tserenchunt Gansukh et al.
    Journal of Histochemistry & Cytochemistry
  8. Granule exocytosis is required for platelet spreading: differential sorting of alpha-granules expressing VAMP-7.
    Blood, 2012-05-15;120(1):199-206.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Conditionally replicating adenovirus expressing TIMP2 for ovarian cancer therapy.
    Authors: Yang SW, Cody JJ, Rivera AA, Waehler R, Wang M, Kimball KJ, Alvarez RA, Siegal GP, Douglas JT, Ponnazhagan S
    Clin. Cancer Res., 2010-11-29;17(3):538-49.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010-01-14;82(5):968-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell-based ELISA
  11. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
    Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
    Exp. Hematol., 2009-05-03;37(7):849-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2008-12-16;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
    Applications: Western Blot
  13. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
    Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2007-05-01;178(9):5957-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  14. Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
    Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
    Gut, 2006-05-08;55(12):1774-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human TIMP-2 Antibody
By Anonymous on 08/14/2019
Application: WB Sample Tested: Recombinant protein Species: Human
Western Blot Human TIMP-2 Antibody MAB971