Human TIMP-3 APC-conjugated Antibody

Catalog #: IC9731A Datasheet / COA / SDS

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IC9731A

Detection of TIMP‑3 in MDA‑MB‑231 Human Cell Line by Flow Cytometry.

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All TIMP-3 Antibodies

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Human TIMP-3 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TIMP-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TIMP-1, -2, or -4 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 277128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIMP-3
Cys24-Pro211
Accession # P35625

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TIMP-3 antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry.

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Detection of TIMP‑3 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TIMP-3 APC-conjugated Monoclonal Antibody (Catalog # IC9731A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TIMP-3

Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE.

TIMP-3 is unique among the TIMPs because of its high affinity for binding to the extracellular matrix. Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.

Long Name

Tissue Inhibitors of Metalloproteinases 3

Entrez Gene IDs

7078 (Human); 21859 (Mouse); 25358 (Rat)

Alternate Names

HSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; pseudoinflammatory); SFD; TIMP metallopeptidase inhibitor 3; TIMP3; TIMP-3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy; Tissue inhibitor of metalloproteinases 3