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Human TIMP-4 Antibody

Catalog #: MAB974
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB974
MAB974-SP
TIMP‑4 in Human Breast Cancer Tissue.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIMP-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIMP-4 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human TIMP-1, -2, -3, or recombinant mouse TIMP-1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 153934
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-4
Cys30-Pro244
Accession # Q99727
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TIMP‑4 (Catalog # 974-TSF)
Immunohistochemistry
8-25 µg/mL
See below

Human TIMP-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human TIMP‑4 Biotinylated Antibody (Catalog # BAF974)

Standard: Recombinant Human TIMP-4 (Sf 21-expressed) Protein, CF (Catalog # 974-TSF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TIMP‑4 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

TIMP‑4 in Human Breast Cancer Tissue. TIMP-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human TIMP-4 Monoclonal Antibody (Catalog # MAB974) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-4

TIMP-4 is a secreted protein that inhibits most MMPs by forming a noncovalent complex with the MMP active site. It is highly expressed in brain, heart, ovary, and skeletal muscle.

Long Name
Tissue Inhibitors of Metalloproteinases 4
Entrez Gene IDs
7079 (Human); 110595 (Mouse)
Alternate Names
metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4

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Citations for Human TIMP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Effect of SPARC Suppression in Mice, Perfused Human Anterior Segments, and Trabecular Meshwork Cells
    Authors: WW MacDonald, SS Swaminatha, JY Heo, A Castillejo, J Hsueh, BJ Liu, D Jo, A Du, H Lee, MH Kang, DJ Rhee
    Investigative Ophthalmology & Visual Science, 2022-06-01;63(6):8.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Glial cell missing-1 mediates over-expression of tissue inhibitor of metalloproteinase-4 in severe pre-eclamptic placental villi.
    Authors: Drewlo S, Czikk M, Baczyk D, Lye S, Kingdom J
    Hum. Reprod., 2011-03-15;26(5):1025-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
    Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
    Exp. Hematol., 2009-05-03;37(7):849-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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