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Human TLR2 Antibody

Catalog #: AF2616
18 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2616
AF2616-SP
Detection of Human TLR2 by Western Blot.
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Product Details
Citations (18)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TLR2 in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant mouse TLR2 and less than 0.3% cross-reactivity with recombinant human (rh) TLR3 and rhTLR4/MD-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TLR2
Glu21-Leu590
Accession # O60603
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human TLR2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human TLR2 Biotinylated Antibody (Catalog # BAF2616)

Standard: Recombinant Human TLR2 Protein, CF (Catalog # 2616-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TLR2 antibody by Western Blot. View Larger

Detection of Human TLR2 by Western Blot. Western blot shows lysate of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TLR2 at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR2

Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1‑3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid (aa) precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell costimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).

References
  1. Wetzler, L. (2003) Vaccine 21:S2/55.
  2. Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121.
  3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  4. Flo T. et al. (2001) J. Leukoc. Biol. 69:474.
  5. Akira S. (2003) Curr. Opin. Immunol. 15:5.
  6. Yamamoto M. et al. (2002) Nature 420:324.
Long Name
Toll-like Receptor 2
Entrez Gene IDs
7097 (Human); 24088 (Mouse); 310553 (Rat)
Alternate Names
CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

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Citations for Human TLR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. PAUF Induces Migration of Human Pancreatic Cancer Cells Exclusively via the TLR4/MyD88/NF-kappa B Signaling Pathway
    Authors: Youn SE, Jiang F, Won HY et al.
    International journal of molecular sciences
  2. Expression and regulation of toll-like receptors (TLRs) in human intervertebral disc cells.
    Authors: Klawitter M, Hakozaki M, Kobayashi H et al.
    Eur Spine J.
  3. Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma
    Authors: Karavyraki M, Porter RK.
    Medical Oncology
  4. Identification of TLR2 Signalling Mechanisms Which Contribute to Barrett’s and Oesophageal Adenocarcinoma Disease Progression
    Authors: Ewelina Flis, Gillian Barber, Ciara Nulty, Brian Keogh, Peter McGuirk, Akanksha Anand et al.
    Cancers (Basel)
  5. CCN1 is an opsonin for bacterial clearance and a direct activator of Toll-like receptor signaling
    Authors: JI Jun, LF Lau
    Nat Commun, 2020-03-06;11(1):1242.
  6. NOS inhibition reverses TLR2-induced chondrocyte dysfunction and attenuates age-related osteoarthritis
    Authors: Shen, P;Serve, S;Wu, P;Liu, X;Dai, Y;Durán-Hernández, N;Nguyen, DTM;Fuchs, M;Maleitzke, T;Reisener, MJ;Dzamukova, M;Nussbaumer, K;Brunner, TM;Li, Y;Holecska, V;Heinz, GA;Heinrich, F;Durek, P;Katsoula, G;Gwinner, C;Jung, T;Zeggini, E;Winkler, T;Mashreghi, MF;Pumberger, M;Perka, C;Löhning, M;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC/IF
  7. Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma
    Authors: Karavyraki M, Porter RK.
    Medical Oncology
  8. LYSMD3: A mammalian pattern recognition receptor for chitin
    Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
    Cell Reports, 2021-07-20;36(3):109392.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Inhibition of osteoclastogenesis by opsonized Porphyromonas gingivalis
    Authors: Subramanya N. Pandruvada, Jeffrey L. Ebersole, Sarandeep S. Huja
    FASEB BioAdvances
  10. Placental inflammation by HMGB1 activation of TLR4 at the syncytium
    Authors: Line H. Tangerås, Gabriela B. Silva, Guro S. Stødle, Lobke M. Gierman, Bente Skei, Karin Collett et al.
    Placenta
  11. Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
    Authors: PJ Huang, PY Low, I Wang, SD Hsu, T Angata
    J. Biol. Chem., 2018-10-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  12. Toll-like receptor 2 is increased in neurons in Parkinson's disease brain and may contribute to alpha-synuclein pathology
    Authors: Glenda M Halliday
    Acta Neuropathol., 2016-11-25;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Metalloproteinase-dependent TLR2 ectodomain shedding is involved in soluble toll-like receptor 2 (sTLR2) production.
    Authors: Langjahr P, Diaz-Jimenez D, De la Fuente M, Rubio E, Golenbock D, Bronfman F, Quera R, Gonzalez M, Hermoso M
    PLoS ONE, 2014-12-22;9(12):e104624.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response
    Authors: Kevin Wilhelmsen, Kailin R Mesa, Arun Prakash, Fengyun Xu, Judith Hellman
    Innate Immunity
  15. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012-06-15;287(32):26478-94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Maternal high-fat diet disturbs uteroplacental hemodynamics and increases the frequency of stillbirth in a nonhuman primate model of excess nutrition.
    Authors: Frias AE, Morgan TK, Evans AE
    Endocrinology, 2011-03-29;152(6):2456-64.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo.
    Authors: Shin HS, Xu F, Bagchi A, Herrup E, Wilhelmsen K
    J. Immunol., 2010-12-17;186(2):1119-30.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Cell-based ELISA, Flow Cytometry, Western Blot
  18. Platelets mediate inflammatory monocyte activation by SARS-CoV-2 Spike protein
    Authors: Li T, Yang Y, Li Y et al.
    The Journal of clinical investigation

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