What is the normal appearance of Assay Diluent RD1-75?

Name: Human TLR2 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DTLR20

Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.

Human TLR2 Quantikine ELISA Kit

Catalog #: DTLR20 Datasheet / COA / SDS

Discontinued Product

DTLR20 has been discontinued.

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Human TLR2 ELISA Serum/Plasma Standard Curve

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Product Details

Procedure

FAQs

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human TLR2 Quantikine ELISA Kit Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Cell Lysates (10 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (34 uL), Urine (100 uL), Human Milk (10 uL)

Sensitivity

0.109 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human TLR2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human TLR2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TLR2 in cell culture supernates, cell lysates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human TLR2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TLR2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TLR2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.32	2.72	5.47	1.4	2.82	5.73
Standard Deviation	0.032	0.055	0.109	0.105	0.225	0.427
CV%	2.4	2	2	7.5	8	7.5

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.43	3.01	5.82	1.48	3.12	6.2
Standard Deviation	0.051	0.065	0.155	0.128	0.31	0.46
CV%	3.6	2.2	2.7	8.6	9.9	7.4

Recovery

The recovery of human TLR2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	92-98
EDTA Plasma (n=4)	98	86-107
Heparin Plasma (n=4)	98	88-105
Serum (n=4)	99	84-111
Urine (n=4)	98	91-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TLR2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human TLR2 ELISA Serum/Plasma Standard Curve

N/A

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Human TLR2 ELISA Cell Culture Supernate/Cell Lysate/ Saliva/Urine/Human Milk Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TLR2

TLRs make up a family of pattern recognition receptors that play important roles in the innate immune response. Broad classes of pathogens (e.g. viruses, bacteria, and fungi) constitutively express a set of mutation-resistant molecules called pathogen-associated molecular patterns (PAMPs). These microbial molecular markers may be composed of proteins, carbohydrates, lipids, nucleic acids and/or combinations thereof. Individual TLRs recognize distinct pathogen-associated PAMPs, initiating signaling cascades that promote the immune response. Structurally, TLRs are type I transmembrane receptors that possess varying numbers of extracellular N-terminal leucine-rich repeat (LRR) motifs, followed by a cysteine-rich region, a TM domain, and an intracellular Toll/IL-1 R (TIR) motif. The TIR motif is common to the larger IL-1 R/TLR superfamily.

Long Name:

Toll-like Receptor 2

Entrez Gene IDs:

7097 (Human); 24088 (Mouse); 310553 (Rat)

Alternate Names:

CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

Related Research Areas

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

100 µL Assay Diluent

Add 100 µL of Assay Diluent to each well.

100 µL Standard, Control, or Sample

Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

200 µL Conjugate

Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
Aspirate and wash 4 times.

200 µL Substrate Solution

Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

50 µL Stop Solution

Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

FAQs

What is the normal appearance of Assay Diluent RD1-75?
- Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.

View all ELISA FAQs