Human TLR9 Antibody Summary
Asn64-Glu189
Accession # Q9NR96
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TLR9 in Human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Sheep Anti-Human TLR9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3658) followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).
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TLR9 in Human Tonsil. TLR9 was detected in immersion fixed paraffin-embedded sections of human tonsil using Sheep Anti-Human TLR9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3658) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR9
TLR9 (Toll receptor 9; also CD289) is a 145-150 kDa member of the Toll-like receptor family of molecules. It is expressed by colonic epithelium, CD123+ plasmacytoid dendritic cells, and transitional B cells, and responds to unmethylated DNA CpG motifs that exhibit either a GTCGTT sequence (in human), or a GACGTT sequence (in mouse). TLR9 is found in the ER, and translocates to either the cell membrane, or to lysosomes where it binds bacterial DNA. Precursor human TLR9 is a type I transmembrane protein 1032 amino acids (aa) in length. It possesses a 793 aa extracellular region that contains 26 LRRs (aa 26-818), plus a 193 aa cytoplasmic domain. The full-length 150 kDa form is suggested to be ligand-binding but nonsignaling. The active form is believed to be an 80 kDa cleavage product found in the endosome compartment. There are multiple splice forms. One contains a deletion of aa 2-16, a second possesses an alternate start site at Met58, while a third and fourth show alternative start sites aa 23 and 24 upstream of the standard site. Over aa 64-189, human TLR9 shares 76% aa identity with mouse TLR9.
Product Datasheets
Citation for Human TLR9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Fc?RIIIa Signaling Modulates Endosomal TLR Responses in Human CD4(+) T Cells
Authors: AK Chauhan
J. Immunol., 2017-05-12;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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