Human TM4SF1/L6 Antibody Summary
Met1-Cys202
Accession # P30408
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TM4SF1/L6 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
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Detection of TM4SF1/L6 in HEK293 Human Cell Line Transfected with Human TM4SF1/L6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TM4SF1/L6 and eGFP was stained with either (A) Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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TM4SF1/L6 in A549 Human Cell Line. TM4SF1/L6 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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TM4SF1/L6 in HepG2 Human Cell Line. TM4SF1/L6 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and HEK293 human embryonic kidney cell line (negative control) using Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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TM4SF1/L6 in Human Breast Cancer Tissue. TM4SF1/L6 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TM4SF1/L6
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TM4SF1 (Transmembrane 4 L6 family Member 1; also L6 and M3s1) is a 23-28 kDa member of the L6 tetraspanin family of molecules. It is expressed by fibroblasts, endothelial cells and a variety of tumor cells. TM4SF1 is embedded in both the plasma membrane and the membrane of late endocytic organelles. Here, it appears to be ubiquitinated, and to regulate endocytosis, an action that impacts effective cell migration. TM4SF1 is reported to interact with alpha 5 and beta 1 integrins, and this may impact cell motility. It also suppresses the expression of CD63 and CD82, two molecules that impede cell mobility. Mouse TM4SF1 is a 202 amino acid (aa) 4‑transmembrane (TM) glycoprotein. It contains a short N-terminal cytoplasmic region (aa 1-9), followed by four TM regions and another C-terminal cytoplasmic region (aa 183-202). There is one potential isoform variant that shows an alternative start site at Met60. Over aa 116-161, mouse TM4SF1 shares 73% and 96% aa sequence identity with human and rat TM4SF1, respectively. |
Product Datasheets
Citations for Human TM4SF1/L6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor
Authors: WJ Zacharias, DB Frank, JA Zepp, MP Morley, FA Alkhaleel, J Kong, S Zhou, E Cantu, EE Morrisey
Nature, 2018-02-28;555(7695):251-255.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity
Authors: Vincent R. Knight-Schrijver, Hongorzul Davaapil, Semih Bayraktar, Alexander D. B. Ross, Kazumasa Kanemaru, James Cranley et al.
Nature Cardiovascular Research
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