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Human TMEM87A Antibody

Catalog #: MAB7966 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7966
MAB7966-SP
Detection of Human TMEM87A by Western Blot.
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Human TMEM87A Antibody Summary

Species Reactivity
Human
Specificity
Detects human TMEM87A in ELISAs and Western Blots
Source
Monoclonal Mouse IgG2B Clone # 772807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TMEM87A
Ser22-Asn157
Accession # Q8NBN3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TMEM87A antibody by Western Blot. View Larger

Detection of Human TMEM87A by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TMEM87A at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of TMEM-87A antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TMEM-87A in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Simple Western Detection of Human TMEM87A antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human TMEM87A by Simple WesternTM. Simple Western lane view shows lysate of T47D human breast cancer cell line, loaded at 0.5 mg/mL. Specific bands were detected for TMEM87A at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEM87A

TMEM87A (Transmembrane protein 87A) is predicted to be a 6-transmembrane, 555 amino acid (aa) glyco- and phospho-protein that is expressed on the cell surface. Its expression in the mammary gland is upregulated when there is a loss of caveolin-1 expression, which may confer susceptibility to breast cancer. Within the region used as an immunogen, human TMEM87A shares 83% aa sequence identity with mouse and rat TMEM87A. A potential isoform of 181 aa diverges C-terminal to aa 169, while another of 494 aa is divergent N-terminal to aa 70.

Long Name
Transmembrane Protein 87A
Entrez Gene IDs
25963 (Human); 211499 (Mouse); 366170 (Rat)
Alternate Names
DKFZp564G2022; FLJ11273; MGC33727; PSEC0094; TMEM87A; transmembrane protein 87A

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