Human TMEM87A APC-conjugated Antibody

Catalog #: FAB7966A Datasheet / COA / SDS

Discontinued Product

FAB7966A has been discontinued.
View all TMEM87A products.
Detection of TMEM87A in LNCaP Human Cell Line by Flow Cytometry.
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Human TMEM87A APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TMEM87A in direct ELISAs and Western Blots
Source
Monoclonal Mouse IgG2B Clone # 772807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TMEM87A
Ser22-Asn157
Accession # Q8NBN3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TMEM87A antibody in LNCaP Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TMEM87A in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human TMEM87A APC-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB7966A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TMEM87A

TMEM87A (Transmembrane protein 87A) is predicted to be a 6-transmembrane, 555 amino acid (aa) glyco- and phospho-protein that is expressed on the cell surface. Its expression in the mammary gland is upregulated when there is a loss of caveolin-1 expression, which may confer susceptibility to breast cancer. Within the region used as an immunogen, human TMEM87A shares 83% aa sequence identity with mouse and rat TMEM87A. A potential isoform of 181 aa diverges C-terminal to aa 169, while another of 494 aa is divergent N-terminal to aa 70.

Long Name
Transmembrane Protein 87A
Entrez Gene IDs
25963 (Human); 211499 (Mouse); 366170 (Rat)
Alternate Names
DKFZp564G2022; FLJ11273; MGC33727; PSEC0094; TMEM87A; transmembrane protein 87A

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