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Human TNF-alpha Antibody

Catalog #: MAB610
70 Citations 4 Reviews Datasheet / COA / SDS
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MAB610-SP
MAB610-100
MAB610-500
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Detection of Recombinant Human TNF‑ alpha by Western Blot.
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Citations (70)
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Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF-alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF‑ beta, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, and recombinant porcine TNF‑ alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 28401
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TNF-alpha
Gly57-Leu233 (predicted)
Accession # P01375
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Human TNF-alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human TNF‑ alpha Biotinylated Antibody (Catalog # BAF210)

Neutralization
Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.01-0.04 µg/mL in the presence of actinomycin D and 0.25 ng/mL Recombinant Human TNF‑ alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human TNF-a antibody by Western Blot. View Larger

Detection of Recombinant Human TNF‑ alpha by Western Blot. Western blot shows 25 ng of Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Rat TNF-a (Catalog # 510-RT), and Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry TNF-a antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

TNF‑ alpha in Human PBMCs. TNF-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody. View Larger

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody. Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610). The ND50 is typically 0.01-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF- is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

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Citations for Human TNF-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

70 Citations: Showing 1 - 10
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  1. Regulation of human glioblastoma cell death by combined treatment of cannabidiol, gamma -radiation and small molecule inhibitors of cell signaling pathways
    Authors: Vladimir N. Ivanov, Jinhua Wu, Tom K. Hei
    Oncotarget
  2. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours
    Authors: Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi et al.
    Transplantation
  3. Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva
    Authors: Arpita Saha, Hamdi Ben Halima, Abhishek Saini, Juan Gallardo-Gonzalez, Nadia Zine, Clara Viñas et al.
    Molecules
  4. TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2
    Authors: Paramita Baruah, Jessica Bullenkamp, Philip O. G. Wilson, Michael Lee, Juan Carlos Kaski, Ingrid E. Dumitriu
    Frontiers in Immunology
  5. Lipopolysaccharide-Stimulated Human Fetal Membranes Induce Neutrophil Activation and Release of Vital Neutrophil Extracellular Traps
    Authors: Mancy Tong, Julie A. Potter, Gil Mor, Vikki M. Abrahams
    The Journal of Immunology
  6. Normal serum levels of immune complexes in postpolio patients
    Authors: Eva Melin, Azita Sohrabian, Johan Rönnelid, Kristian Borg
    Results in Immunology
  7. UL16-Binding Protein 1 Induced HTR-8/SVneo Autophagy via NF-kappa B Suppression Mediated by TNF-alpha Secreted through uNK Cells
    Authors: Jing Liu, Guang Song, Tao Meng, Ge Zhao
    BioMed Research International
  8. AZD7624, an Inhaled p38 Inhibitor, Demonstrates Local Lung Inhibition of LPS-Induced TNF alpha with Minimal Systemic Exposure
    Authors: Rikard Pehrson, Tove Hegelund-Myrbäck, Danen Cunoosamy, Sara Asimus, Paul Jansson, Naimish Patel et al.
    Journal of Pharmacology and Experimental Therapeutics
  9. The Role of Historical Bioactivity Data in the Deconvolution of Phenotypic Screens
    Authors: Aurelie Bornot, Carolyn Blackett, Ola Engkvist, Clare Murray, Claus Bendtsen
    J Biomol Screen
  10. Progranulin Does Not Bind Tumor Necrosis Factor (TNF) Receptors and Is Not a Direct Regulator of TNF-Dependent Signaling or Bioactivity in Immune or Neuronal Cells.
    Authors: Chen X, Chang J, Deng Q et al.
    J Neurosci.
  11. Alloreactive fetal T cells promote uterine contractility in preterm labor via IFN-gamma and TNF-alpha
    Authors: Michela Frascoli, Lacy Coniglio, Russell Witt, Cerine Jeanty, Shannon Fleck-Derderian, Dana E. Myers et al.
    Science Translational Medicine
  12. La infección con el virus del dengue induce apoptosis en células del neuroblastoma humano SH-SY5Y
    Authors: Jaime E. Castellanos, José I. Neissa, Sigrid J. Camacho
    Biomédica
  13. Application of the Mirrorball High-Sensitivity Cytometer to Multiplexed Assays for Antibody Drug Discovery
    Authors: Elizabeth England, Philip Newton, Frances Neal, Lisa Kitching, Caroline Colley, Christine J. Rossant
    J Biomol Screen
  14. CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy
    Authors: Abu El-Asrar, AM;Nawaz, MI;Ahmad, A;Dillemans, L;Siddiquei, M;Allegaert, E;Gikandi, PW;De Hertogh, G;Opdenakker, G;Struyf, S;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Mycoplasma hyorhinis infection promotes TNF-? signaling and SMAC mimetic-mediated apoptosis in human prostate cancer
    Authors: Kim, JK;Chang, I;Jung, Y;Kaplan, Z;Hill, EE;Taichman, RS;Krebsbach, PH;
    Heliyon
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  16. Loss of Function TGFBR2 Variant as a Contributing Factor in Generalized Pustular Psoriasis and Adult-Onset Immunodeficiency
    Authors: P Kantaputra, T Daroontum, M Chuamanoch, S Chaowattan, W Intachai, B Olsen, T Sastraruji, S Tongsima, C Ngamphiw, J Kampuansai, TC Cox, S Kiratikano
    Genes, 2022-12-29;14(1):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. The secreted protein Cowpox Virus 14 contributes to viral virulence and immune evasion by engaging Fc-gamma-receptors
    Authors: RF Iyer, DM Edwards, P Kolb, HP Raué, CA Nelson, ML Epperson, MK Slifka, JC Nolz, H Hengel, DH Fremont, K Früh
    PloS Pathogens, 2022-09-19;18(9):e1010783.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  18. TNFalpha Mediates Inflammation-Induced Effects on PPARG Splicing in Adipose Tissue and Mesenchymal Precursor Cells
    Authors: S Cataldi, M Aprile, D Melillo, I Mucel, S Giorgetti-, M Cormont, P Italiani, M Blüher, JF Tanti, A Ciccodicol, V Costa
    Cells, 2021-12-24;11(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. The effect of Gestational Diabetes Mellitus on the fetal compartment
    Authors: TPB De Luccia, E Ono, R Menon, AU Borbely, R Mattar, L Richardson, ALM da Silva, RM Botelho, MLTLF da Rocha, S Daher
    Journal of reproductive immunology, 2021-03-24;145(0):103314.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. The circular RNA landscape in specific peripheral blood mononuclear cells of critically ill patients with sepsis
    Authors: HN Khan, X Brands, S Aufiero, AJ Hoogendijk, AM Klarenbeek, TSR van Engele, BW Haak, LA van Vught, J Horn, MJ Schultz, AH Zwinderman, T van der Po, BP Scicluna
    Crit Care, 2020-07-13;24(1):423.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Shikonin blocks human lung adenocarcinoma cell migration and invasion in the inflammatory microenvironment via the IL?6/STAT3 signaling pathway
    Authors: T Pan, F Zhang, F Li, X Gao, Z Li, X Li, X Ren
    Oncol. Rep., 2020-07-09;44(3):1049-1063.
    Species: Human
    Sample Types: Cells, Whole Cells
    Applications: B/N, Neutralization
  22. Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex
    Authors: J Seo, D Seong, YW Nam, CH Hwang, SR Lee, CS Lee, Y Jin, HW Lee, DB Oh, P Vandenabee, J Song
    Cell Death Differ., 2020-05-26;0(0):.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  23. Targeting triple-negative breast cancers with the Smac-mimetic birinapant
    Authors: N Lalaoui, D Merino, G Giner, F Vaillant, D Chau, L Liu, T Kratina, B Pal, JR Whittle, N Etemadi, J Berthelet, J Gräsel, C Hall, ME Ritchie, M Ernst, GK Smyth, DL Vaux, JE Visvader, GJ Lindeman, J Silke
    Cell Death Differ., 2020-04-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
    Authors: A Stumpf, T Brandstett, J Hübner, J Rühe
    PLoS ONE, 2019-12-02;14(12):e0225525.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  25. Epithelial HMGB1 Delays Skin Wound Healing and Drives Tumor Initiation by Priming Neutrophils for NET Formation
    Authors: E Hoste, C Maueröder, L van Hove, L Catrysse, HK Vikkula, M Sze, B Maes, D Karjosukar, L Martens, A Gonçalves, E Parthoens, R Roelandt, W Declercq, I Fuentes, F Palisson, S Gonzalez, JC Salas-Alan, L Boon, P Huebener, KW Mulder, K Ravichandr, Y Saeys, RF Schwabe, G van Loo
    Cell Rep, 2019-11-26;29(9):2689-2701.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation
    Authors: W Sun, N Meednu, A Rosenberg, J Rangel-Mor, V Wang, J Glanzman, T Owen, X Zhou, H Zhang, BF Boyce, JH Anolik, L Xing
    Nat Commun, 2018-12-03;9(1):5127.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Dysfunctional endothelial-derived microparticles promote inflammatory macrophage formation via NF-?B and IL-1? signal pathways
    Authors: Y Wang, J Liu, X Chen, H Sun, S Peng, Y Kuang, J Pi, T Zhuang, L Zhang, Z Yu, B Tomlinson, P Chan, Y Chen, Y Zhang, Y Li
    J. Cell. Mol. Med., 2018-10-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. The induced RNA-binding protein, HuR targets 3'-UTR region of IL-6 mRNA and enhances its stabilization in periodontitis
    Authors: K Ouhara, S Munenaga, M Kajiya, K Takeda, S Matsuda, Y Sato, Y Hamamoto, T Iwata, S Yamasaki, K Akutagawa, N Mizuno, T Fujita, E Sugiyama, H Kurihara
    Clin. Exp. Immunol., 2018-03-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Effects of anti-inflammatory drugs on the expression of tryptophan-metabolism genes by human macrophages
    Authors: T Regan, AC Gill, SM Clohisey, MW Barnett, CM Pariante, NA Harrison, DA Hume, ET Bullmore, TC Freeman
    J. Leukoc. Biol., 2018-01-26;103(4):681-692.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis?
    Authors: S Urra, MC Fischer, JR Martínez, L Véliz, P Orellana, A Solar, K Bohmwald, A Kalergis, C Riedel, AH Corvalán, JC Roa, R Fuentealba, CJ Cáceres, M López-Last, A León, N Droppelman, HE González
    Oncotarget, 2017-12-20;9(2):2445-2467.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  31. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017-02-16;65(4):715-729.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Combination of IAP antagonist and IFN? activates novel caspase-10- and RIPK1-dependent cell death pathways
    Authors: MC Tanzer, N Khan, JA Rickard, N Etemadi, N Lalaoui, SK Spall, JM Hildebrand, D Segal, M Miasari, D Chau, WL Wong, M McKinlay, SK Chunduru, CA Benetatos, SM Condon, JE Vince, MJ Herold, J Silke
    Cell Death Differ, 2017-01-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. Lysine fatty acylation promotes lysosomal targeting of TNF-?
    Authors: H Jiang, X Zhang, H Lin
    Sci Rep, 2016-04-15;6(0):24371.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  34. Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcgammaR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment.
    Authors: Matt P, Lindqvist U, Kleinau S
    PLoS ONE, 2015-09-25;10(9):e0137474.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  35. Increased CD160 expression on circulating natural killer cells in atherogenesis.
    Authors: Zuo J, Shan Z, Zhou L, Yu J, Liu X, Gao Y
    J Transl Med, 2015-06-13;13(0):188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  36. Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
    Authors: Tudor C, Bruns H, Daniel C, Distel L, Hartmann A, Gerbitz A, Buettner M
    PLoS ONE, 2014-12-03;9(12):e114345.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  37. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013-10-12;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  38. The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.
    Authors: Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M
    Clin Cancer Res, 2013-02-12;19(7):1784-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
    Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
    Cancer Res., 2011-11-07;72(1):66-75.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  40. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011-07-09;54(10):2713-23.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  41. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011-02-23;186(7):4110-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  42. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  43. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  44. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  45. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  46. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
    Authors: Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
    J. Immunol., 2008-02-15;180(4):2553-61.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  47. Interleukin-10 modulates pro-apoptotic effects of TNF-alpha in human articular chondrocytes in vitro.
    Authors: John T, Muller RD, Oberholzer A, Zreiqat H, Kohl B, Ertel W, Hostmann A, Tschoeke SK, Schulze-Tanzil G
    Cytokine, 2007-11-19;40(3):226-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  48. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007-11-01;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  49. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
    Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
    J. Clin. Oncol., 2007-10-10;25(29):4542-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  50. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  51. Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis.
    Authors: Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M
    J. Pathol., 2007-05-01;212(1):47-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  52. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  53. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  54. Selective regulation of IL-10 signaling and function by zymosan.
    Authors: Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB
    J. Immunol., 2006-04-15;176(8):4785-92.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  55. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
    Applications: ELISA Development
  56. Sequential induction of angiogenic growth factors by TNF-alpha in choroidal endothelial cells.
    Authors: Hangai M, He S, Hoffmann S, Lim JI, Ryan SJ, Hinton DR
    J. Neuroimmunol., 2005-11-08;171(1):45-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  57. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  58. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004-06-01;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  59. IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
    Authors: Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB
    J. Immunol., 2004-05-15;172(10):6476-81.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  60. True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha?
    Authors: Buch MH, Conaghan PG, Quinn MA, Bingham SJ, Veale D, Emery P
    Ann. Rheum. Dis., 2004-03-19;63(10):1344-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  61. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  62. Reprogramming of IL-10 activity and signaling by IFN-gamma.
    Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
    J. Immunol., 2003-11-15;171(10):5034-41.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  63. Dual-Enhanced Plasmonic Biosensing for Point-of-Care Sepsis Detection
    Authors: Lip Ket Chin, Jun-Yeong Yang, Benjamin Chousterman, Sunghoon Jung, Do-Geun Kim, Dong-Ho Kim et al.
    ACS Nano
  64. Autophagy-based unconventional secretion of HMGB1 in glioblastoma promotes chemosensitivity to temozolomide through macrophage M1-like polarization
    Authors: Li Z, Fu WJ, Chen XQ et al.
    Journal of experimental & clinical cancer research : CR
  65. Efficacy of birinapant in combination with carboplatin in targeting platinum‑resistant epithelial ovarian cancers
    Authors: Tanya Singh, Adam Neal, Gabriella Dibernardo, Neela Raheseparian, Neda A. Moatamed, Sanaz Memarzadeh
    International Journal of Oncology
  66. Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips
    Authors: Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille
    BMC Biotechnology
  67. Inflammation-related cytokines and their roles in gastroenteropancreatic neuroendocrine neoplasms
    Authors: Davorka Herman Herman Mahečić, Maja Cigrovski Cigrovski Berković, Vanja Zjačić-Rotkvić, Tamara Čačev, Sanja Kapitanović, Monika Ulamec
    Bosnian Journal of Basic Medical Sciences
  68. Simultaneous Immunoassay Analysis of Plasma IL-6 and TNF-alpha on a Microchip
    Authors: Kaori Abe, Yoshiko Hashimoto, Shouki Yatsushiro, Shohei Yamamura, Mika Bando, Yuka Hiroshima et al.
    PLoS ONE
  69. Inhibition of matrix metalloproteinase-9 secretion by dimethyl sulfoxide and cyclic adenosine monophosphate in human monocytes
    Authors: Darcy R Denner, Maria LD Udan-Johns, Michael R Nichols
    World Journal of Biological Chemistry
  70. Multiplexed Protein Quantification with Barcoded Hydrogel Microparticles
    Authors: David C. Appleyard, Stephen C. Chapin, Patrick S. Doyle
    Analytical Chemistry

FAQs

  1. Can catalog # MAB610 neutralize mouse TNF-alpha?

    • MAB610 shows less than 0.05% cross-reactivity with recombinant mouse TNF-alpha and does not neutralize mouse TNF-alpha. 

       

  2. What is the light chain of Human TNF-alpha Antibody, Catalog # MAB610, Clone # 28401?

    • Catalog # MAB610 has a kappa light chain.

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Human TNF-alpha Antibody
By Anonymous on 06/16/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: macrophages Species: Human
Immunocytochemistry/Immunofluorescence Human TNF-alpha Antibody MAB610
Human TNF-alpha Antibody
By Anonymous on 05/23/2019
Application: ELISA Sample Tested: Heparin Plasma Species: Human
ELISA Human TNF-alpha Antibody MAB610
Human TNF-alpha Antibody
By Anonymous on 08/20/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human
ELISA Human TNF-alpha Antibody MAB610
Human TNF-alpha Antibody
By Anonymous on 11/16/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

MAB610 was used as the capture antibody in an ELISA targeting TNFa in human samples. The antibody AF-210 was used as the detection.

ELISA Human TNF-alpha Antibody MAB610