Human TNF-alpha Antibody Summary
Val77-Leu233
Accession # P01375
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody. Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF-a Polyclonal Antibody (Catalog # AB-210-NA). The ND50 is typically 0.05-0.25 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF-alpha
Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF- is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.
Product Datasheets
Citations for Human TNF-alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Supernatants from lymphocytes stimulated with Bacillus Calmette-Guerin can modify the antigenicity of tumours and stimulate allogeneic T-cell responses
Authors: W M Liu, D W Fowler, A M Gravett, P Smith, A G Dalgleish
British Journal of Cancer
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Strain-Modulated and Nanorod-Waveguided Fluorescence in Single Zinc Oxide Nanorod-Based Immunodetection
Authors: Sytu, MRC;Stoner, A;Hahm, JI;
Biosensors
Species: N/A
Sample Types: Small Molecule
Applications: ELISA Detection -
Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.
Authors: Liang X, Moseman EA, Farrar MA
Blood, 2010-03-25;115(24):5041-52.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Antigenic stimulation in the simian model of HIV infection yields dilated cardiomyopathy through effects of TNFalpha.
Authors: Yearley JH, Mansfield KG, Carville AA, Sokos GG, Xia D, Pearson CB, Shannon RP
AIDS, 2008-03-12;22(5):585-94.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Peritonitis in the baboon: a primate model which stimulates human sepsis.
Authors: Kinasewitz GT, 2016, Chang AC, Peer GT, Hinshaw LB, Taylor FB
2000-02-01;13(2):100-9.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Plasma
Applications: ELISA Development -
Ultratrace level determination and quantitative analysis of kidney injury biomarkers in patient samples attained by zinc oxide nanorods
Authors: Manpreet Singh, Anginelle Alabanza, Lorelis E. Gonzalez, Weiwei Wang, W. Brian Reeves, Jong-in Hahm
Nanoscale
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Praliciguat inhibits progression of diabetic nephropathy in ZSF1 rats and suppresses inflammation and apoptosis in human renal proximal tubular cells
Authors: Guang Liu, Courtney M. Shea, Juli E. Jones, Gavrielle M. Price, William Warren, Elisabeth Lonie et al.
American Journal of Physiology-Renal Physiology
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Erythropoietin Protects Adult Retinal Ganglion Cells against NMDA-, Trophic Factor Withdrawal-, and TNF-alpha -Induced Damage
Authors: Zhi-Yang Chang, Ming-Kung Yeh, Chiao-Hsi Chiang, Yi-Hao Chen, Da-Wen Lu
PLoS ONE
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Plasmacytoid dendritic cells are inefficient in activation of human regulatory T cells.
Authors: Hubo M et al.
PLoS One
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