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Human TNF-alpha Antibody

Catalog #: AF-210-NA
41 Citations 3 Reviews Datasheet / COA / SDS
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AF-210-NA
AF-210-SP
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Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF-alpha Antibody.
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Citations (41)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF-alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF‑ alpha in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TNF-alpha
Val77-Leu233
Accession # P01375
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.01-0.06 μg/ml in the presence of 1.5 ng/mL Recombinant Human TNF‑ alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF-alpha Antibody. View Larger

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA). The ND50 is typically 0.01-0.06 µg/mL.

Immunocytochemistry TNF-a antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

TNF‑ alpha in Human PBMCs. TNF-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Functional Detection of Human TNF-alpha by Functional View Larger

Detection of Human TNF-alpha by Functional TPM1 and TPM4 expression in response to TNF alpha.High molecular weight tropomyosin isoform expression in THP1 cells in response to elevated concentrations of TNF alpha. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27649540), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF- is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

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Citations for Human TNF-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. Rapid Downregulation of Innate Immune Cells, Interleukin-12 and Interleukin-23 in Generalized Pustular Psoriasis with Infliximab in Combination with Acitretin
    Authors: M.M. Tang, Z. Spanou, H. Tang, F. Schibler, N. Pelivani, N. Yawalkar
    Dermatology
  2. Changes in podocyte TRPC channels evoked by plasma and sera from patients with recurrent FSGS and by putative glomerular permeability factors
    Authors: Eun Young Kim, Hila Roshanravan, Stuart E. Dryer
    Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
  3. NF-kappa B non-cell-autonomously regulates cancer stem cell populations in the basal-like breast cancer subtype
    Authors: Mizuki Yamamoto, Yuu Taguchi, Taku Ito-Kureha, Kentaro Semba, Noritaka Yamaguchi, Jun-ichiro Inoue
    Nature Communications
  4. TNFR2 depletion reduces psoriatic inflammation in mice via downregulating specific dendritic cell populations in lymph nodes and inhibiting IL-23/IL-17 pathways
    Authors: Unnikrishnan M. Chandrasekharan, Raminderjit Kaur, Jennifer E. Harvey, Chad Braley, Vandana Rai, MacKenzie Lee et al.
    Journal of Investigative Dermatology
  5. Elevated GM-CSF and IL-1beta levels compromise the ability of p38 MAPK inhibitors to modulate TNFalpha levels in the human monocytic/macrophage U937 cell line
    Authors: Christopher W. Espelin, Arthur Goldsipe, Peter K. Sorger, Douglas A. Lauffenburger, David de Graaf, Bart S. Hendriks
    Molecular BioSystems
  6. A Patient with Erythema Nodosus Leprosum and Chagas Cardiopathy: Challenges in Patient Management and Review of the Literature
    Authors: Maria Ângela B. Trindade, Noemia B Carvalho, Maria A. Shikanai-Yasuda, Erika Pagliari, Noemia B. Carvalho, Neusa Y Sakai-Valente et al.
    The American Journal of Tropical Medicine and Hygiene
  7. TNF-Alpha in the Locomotor System beyond Joints: High Degree of Involvement in Myositis in a Rabbit Model
    Authors: Sture Forsgren, Lina Renström, Craig Purdam, James E. Gaida
    International Journal of Rheumatology
  8. Death receptor pathways mediate targeted and non-targeted effects of ionizing radiations in breast cancer cells
    Authors: Audrey Luce, Aurélie Courtin, Céline Levalois, Sandrine Altmeyer-Morel, Paul-Henri Romeo, Sylvie Chevillard et al.
    Carcinogenesis
  9. Intravascular heavy chain-modification of hyaluronan during endotoxic shock
    Authors: K Ni, A Gill, D Cao, K Koike, KS Schweitzer, S Garantziot, I Petrache
    Biochem Biophys Rep, 2018-12-26;17(0):114-121.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  10. Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer
    Authors: M Kim, S Pyo, CH Kang, CO Lee, HK Lee, SU Choi, CH Park
    PLoS ONE, 2018-06-06;13(6):e0198347.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Detection of aryl hydrocarbon receptor agonists in human samples
    Authors: V Rothhammer, DM Borucki, JE Kenison, P Hewson, Z Wang, R Bakshi, DH Sherr, FJ Quintana
    Sci Rep, 2018-03-21;8(1):4970.
    Species: Human
    Sample Types: Serum
    Applications: Neutralization
  12. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
    Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
    Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):.
    Applications: Affinity Assay
  13. Promising Role of Toll-Like Receptor 8 Agonist in Concert with Prostratin for Activation of Silent HIV
    Authors: M A Rochat
    J. Virol, 2017-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients
    PLoS ONE, 2016-09-20;11(9):e0162908.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
    Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
    Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013-10-12;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  17. Neuroblastoma-derived TGF-beta1 modulates the chemokine receptor repertoire of human resting NK cells.
    Authors: Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias M, Moretta A, Bottino C
    J Immunol, 2013-04-10;190(10):5321-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman NC, Stutzman N, Zou Z, Sun PD
    J Leukoc Biol, 2011-11-01;91(2):299-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
    Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
    Haematologica, 2011-05-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
    Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
    J. Immunol., 2011-03-02;186(7):4175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein.
    Authors: Hasegawa H, Thomas HJ, Schooley K, Born TL
    Cytokine, 2011-01-01;53(1):74-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
    Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
    Blood, 2010-06-10;116(12):2061-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  23. Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.
    Authors: Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW
    J. Exp. Med., 2009-08-03;206(9):1883-97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
    Authors: Navenot JM, Fujii N, Peiper SC
    Mol. Pharmacol., 2009-02-06;75(5):1074-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-23;293(1):L69-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
    Authors: Lee SY, Cherla RP, Tesh VL
    Infect. Immun., 2006-12-28;75(3):1291-302.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  27. Spatial compartmentalization of tumor necrosis factor (TNF) receptor 1-dependent signaling pathways in human airway smooth muscle cells. Lipid rafts are essential for TNF-alpha-mediated activation of RhoA but dispensable for the activation of the NF-kappaB and MAPK pathways.
    Authors: Hunter I, Nixon GF
    J. Biol. Chem., 2006-09-18;281(45):34705-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Sepsis and pathophysiology of anthrax in a nonhuman primate model.
    Authors: Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S
    Am. J. Pathol., 2006-08-01;169(2):433-44.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Plasma
    Applications: ELISA Development
  29. Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104.
    Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D
    J. Leukoc. Biol., 2005-06-03;78(2):359-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
    Authors: Andersson By U, Tani E, Andersson U
    J. Pediatr. Hematol. Oncol., 2004-11-01;26(11):706-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004-09-01;80(2):231-5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  32. Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
    Authors: Park JS, 107290, Kim YS, Jee YK, Myong NH, Lee KY
    Active immunotherapy against pathogenic Cis pT231-tau suppresses neurodegeneration in traumatic brain injury mouse models, 2003-05-01;57(5):463-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells.
    Authors: Edgeworth JD, Spencer J, Phalipon A, Griffin GE, Sansonetti PJ
    Eur. J. Immunol., 2002-05-01;32(5):1464-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokines
    Authors: Jennifer Becker, Volker Kinast, Marius Döring, Christoph Lipps, Veronica Duran, Julia Spanier et al.
    Virulence
  35. Sterile inflammation of endothelial cell-derived apoptotic bodies is mediated by interleukin-1 alpha
    Authors: Yaël Berda-Haddad, Stéphane Robert, Paul Salers, Leila Zekraoui, Catherine Farnarier, Charles A. Dinarello et al.
    Proceedings of the National Academy of Sciences
  36. Pathological and ultrastructural analysis of surgical lung biopsies in patients with swine-origin influenza type A/H1N1 and acute respiratory failure
    Authors: Vera Luiza Capelozzi, Edwin Roger Parra, Manoel Ximenes, Ricardo Helbert Bammann, Carmen Silvia Valente Barbas, Marid Irmd Seixas Duarte
    Clinics (Sao Paulo)
  37. Cytokines and Cytokine Receptors Involved in the Pathogenesis of Alzheimer's Disease
    Authors: Tomone Nagae, Kiho Araki, Yuki Shimoda, Lucia I. Sue, Thomas G. Beach, Yoshihiro Konishi
    Journal of Clinical & Cellular Immunology
  38. Neutrophil Necroptosis Is Triggered by Ligation of Adhesion Molecules following GM-CSF Priming
    Authors: Xiaoliang Wang, Zhaoyue He, He Liu, Shida Yousefi, Hans-Uwe Simon
    The Journal of Immunology
  39. A TNF Receptor 2 Selective Agonist Rescues Human Neurons from Oxidative Stress-Induced Cell Death
    Authors: Roman Fischer, Olaf Maier, Martin Siegemund, Harald Wajant, Peter Scheurich, Klaus Pfizenmaier
    PLoS ONE
  40. Blockade of TNF alpha to Improve Human CD34+ Cell Repopulating Activity in Allogeneic Stem Cell Transplantation
    Authors: Vitalyi Senyuk, Pritesh Patel, Nadim Mahmud, Damiano Rondelli
    Frontiers in Immunology
  41. 3D ultrastructural analysis of alpha ‐granule, dense granule, mitochondria, and canalicular system arrangement in resting human platelets
    Authors: Irina D. Pokrovskaya, Shilpi Yadav, Amith Rao, Emma McBride, Jeffrey A. Kamykowski, Guofeng Zhang et al.
    Research and Practice in Thrombosis and Haemostasis

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Reviews for Human TNF-alpha Antibody

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Human TNF-alpha Antibody
By Stephanie Zandee on 04/13/2018
Application: IHC Sample Tested: Adult brain Species: Human

Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).

Immunohistochemistry Human TNF-alpha Antibody AF-210-NA
Human TNF-alpha Antibody
By Balaji Mahender on 01/18/2018
Application: ELISA Sample Tested: Citrate Plasma,EDTA Plasma,Heparin Plasma Species: Human
Human TNF-alpha Antibody
By Anonymous on 11/16/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used with MAB610 to build an ELISA to measure TNFa. This antibody was used as the detection while MAB610 was used as the capture.

ELISA Human TNF-alpha Antibody AF-210-NA