Human TopBP1 Antibody

Catalog #: AF7120 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7120
AF7120-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TopBP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TopBP1 in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TopBP1
Met88-Asp194
Accession # Q92547

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TopBP1 in Human Breast. TopBP1 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human TopBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7120) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TopBP1

TopBP1 (DNA topoisomerase 2-binding protein 1) is a 175-180 kDa member of a critical DNA replication repair complex. It is widely expressed, and appears to serve as an intermediary in DNA replication. When a replication fork is stalled during S phase, DNA continues to unwind ahead of the stall. This becomes associated with RPA, which is essential for ATR and subsequent Chk1 activation. TopBP1 is key to ATR activation, and appears to first bind to DNA:pol-alpha, followed by a 9-1-1 complex and Rad17 interaction that leads to ATR stimulation. Human TopBP1 is 1522 amino acids (aa) in length. It contains eight BRCT domains (aa 101-1486) that interact with distinct binding partners. BRCT1 and 2 binds to Rad9 (aa 101-294), while BRCT 6 and 7 are involved in ATR activation. There are 12 utilized phosphorylation sites and two nuclear localization signals. Three isoform variants have been described. One contains an alternative start site at Met88, a second shows an 18 aa substitution for aa 1198-1522, and a third possesses a six aa substitution for aa 1292-1522. Over aa 88-194, human TopBP1 shares 88% aa identity with mouse TopBP1.

Long Name

DNA Topoisomerase II Binding Protein 1

Entrez Gene IDs

11073 (Human); 235559 (Mouse); 315969 (Rat)

Alternate Names

DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1; KIAA0259DNA topoisomerase 2-binding protein 1; TOP2BP1; TopBP1; topoisomerase (DNA) II binding protein 1