Home / TREML1/TLT-1 / Human TREML1/TLT-1 Antibody

Human TREML1/TLT-1 Antibody

Catalog #: AF2394
1 Citation 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2394
AF2394-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TREML1/TLT-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse TREML1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TREML1/TLT‑1
Gln16-Ser160
Accession # Q86YW5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TREML1/TLT‑1
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood platelets
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML1/TLT-1

TREML1 is a type I transmembrane protein that differs from TREM in the cytoplasmic region by having two ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes. The extracellular region of human and mouse TREML1 share 75% amino acid sequence identity.

Long Name
Triggering Receptor Expressed on Myeloid Cells-like 1
Entrez Gene IDs
340205 (Human); 71326 (Mouse)
Alternate Names
dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human TREML1/TLT-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of thrombin-induced platelet aggregation using human single-chain Fv antibodies specific for TREM-like transcript-1.
    Authors: Giomarelli B, Washington VA, Chisholm MM, Quigley L, McMahon JB, Mori T, McVicar DW
    Thromb. Haemost., 2007-06-01;97(6):955-63.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human TREML1/TLT-1 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human TREML1/TLT-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human TREML1/TLT-1 Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Lymphocytes Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. After primary antibody staining, cells were washED 2x and Alexa 488 labeled rabbit anti-goat IgG (1:200) was added for 20 min. Cells were washed 2x and fixed in orthofix. Isotype control shaded grey, TREML1 in open histogram.
Buffer: 2% FCS, 0.5 mM EDTA
Dilution: 1/100

Flow Cytometry Human TREML1/TLT-1 Antibody AF2394
Human TREML1/TLT-1 Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Blood monocytes Species: Human

Gated on monocytic FSC-A and SSC-A population. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. After primary antibody staining, cells were washed 2x and Alexa 488 labeled rabbit anti-goat IgG (1:200) was added for 20 min. Cells were washed 2x and fixed in orthofix. Isotype control shaded grey, TREML1 in open histogram.
Buffer: 2% HS, 0.5 mM EDTA
Dilution: 1/100

Flow Cytometry Human TREML1/TLT-1 Antibody AF2394