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Human Troponin T (Cardiac) Antibody

Catalog #: MAB1874
17 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1874
MAB1874-SP
Troponin T in BG01V Human Embryonic Stem Cells.
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Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Troponin T (Cardiac) Antibody Summary

Species Reactivity
Human
Specificity
Detects purified human Troponin T (Cardiac) in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 200805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human cardiac Troponin T
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Troponin T antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Troponin T in BG01V Human Embryonic Stem Cells. Troponin T was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry Troponin T antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

Troponin T in Human Heart. Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin T

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Entrez Gene IDs
7139 (Human)
Alternate Names
Cardiac Muscle Troponin T; Cardiomyopathy, Dilated 1D (Autosomal Dominant); Cardiomyopathy, Hypertrophic; CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TNNT2; TnTC; troponin T type 2 (cardiac); Troponin T; troponin T, cardiac muscle; Troponin T2, Cardiac Type; Truncated Cardiac Troponin T

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Citations for Human Troponin T (Cardiac) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Canonical Wnt signaling directs the generation of functional human PSC-derived atrioventricular canal cardiomyocytes in bioprinted cardiac tissues
    Authors: Ye, C;Yang, C;Zhang, H;Gao, R;Liao, Y;Zhang, Y;Jie, L;Zhang, Y;Cheng, T;Wang, Y;Ren, J;
    Cell stem cell
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Immunocytochemistry
  2. Generation of human elongating multi-lineage organized cardiac gastruloids
    Authors: Zachary T. Olmsted, Maria Belen Paredes-Espinosa, Janet L. Paluh
    STAR Protocols
  3. A combined human gastruloid model of cardiogenesis and neurogenesis
    Authors: Olmsted ZT, Paluh JL.
    iScience
  4. Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1
    Authors: YH Chen, PS Huang, MH Wen, M Pan, DF Lee, TY Chen
    Oncogene, 2022-06-14;63(0):102845.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. hESC derived cardiomyocyte biosensor to detect the different types of arrhythmogenic properties of drugs
    Authors: R Pivato, S Klimovic, D Kabanov, F Sverák, M Pesl, J Pribyl, V Rotrekl
    Oncogene, 2022-05-20;1216(0):339959.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes
    Authors: Emmi Helle, Minna Ampuja, Alexandra Dainis, Laura Antola, Elina Temmes, Erik Tolvanen et al.
    Frontiers in Cell and Developmental Biology
  7. Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
    Authors: PS Huang, MH Wen, X Xie, A Xu, DF Lee, TY Chen
    Stem Cell Research, 2021-06-06;54(0):102415.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease
    Authors: J Jung, S Hwang, H Seol, AH Kim, HM Lee, JJ Sung, SM Jeong, YM Choi, JK Jun, DW Kim, J Jang
    Stem Cell Research, 2021-04-10;53(0):102321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  9. Puerarin inhibits apoptosis and inflammation in myocardial cells via PPAR alpha expression in rats with chronic heart failure
    Authors: Le He, Tong Wang, Bing‑Wei Chen, Feng‑Min Lu, Jing Xu
    Experimental and Therapeutic Medicine
  10. Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity
    Authors: Meng Zhao, Yawen Tang, Patrick J. Ernst, Asher Kahn-Krell, Chengming Fan, Danielle Pretorius et al.
    Journal of Visualized Experiments
  11. Quantification of Etoposide Hypersensitivity: A Sensitive, Functional Method for Assessing Pluripotent Stem Cell Quality
    Authors: Frank J. Secreto, Xing Li, Alyson J. Smith, Elizabeth S. Bruinsma, Ester Perales-Clemente, Saji Oommen et al.
    Stem Cells Translational Medicine
  12. Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells
    Authors: F Pei, J Jiang, S Bai, H Cao, L Tian, Y Zhao, C Yang, H Dong, Y Ma
    Stem Cell Res, 2017-01-12;19(0):94-103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  13. Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage
    Authors: Nicolas Christoforou, Syandan Chakraborty, Robert D. Kirkton, Andrew F. Adler, Russell C. Addis, Kam W. Leong
    Scientific Reports
  14. Transcription Factors MYOCD, SRF, Mesp1 and SMARCD3 Enhance the Cardio-Inducing Effect of GATA4, TBX5, and MEF2C during Direct Cellular Reprogramming
    Authors: Nicolas Christoforou, Malathi Chellappan, Andrew F. Adler, Robert D. Kirkton, Tianyi Wu, Russell C. Addis et al.
    PLoS ONE
  15. Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals.
    Authors: Zhang Q, Jiang J, Han P, Yuan Q, Zhang J, Zhang X, Xu Y, Cao H, Meng Q, Chen L, Tian T, Wang X, Li P, Hescheler J, Ji G, Ma Y
    Cell Res., 2010-11-23;21(4):579-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  16. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
    Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
    Cardiovasc. Res., 2010-07-29;89(1):157-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Stem cells derived from human fetal membranes display multilineage differentiation potential.
    Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
    Biol. Reprod., 2007-05-09;77(3):577-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human Troponin T (Cardiac) Antibody

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Human Troponin T (Cardiac) Antibody
By Anonymous on 07/04/2018
Application: Flow Sample Tested: iPSC-CMs Species: Human
Flow Cytometry Human Troponin T (Cardiac) Antibody MAB1874
Human Troponin T (Cardiac) Antibody
By Harsha Vardhan Renikunta on 05/19/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Human iPSC-Derived Cardiomyocytes Species: Human
Immunocytochemistry/Immunofluorescence Human Troponin T (Cardiac) Antibody MAB1874
Human Troponin T (Cardiac) Antibody
By Anonymous on 12/15/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Induced Pluripotent Stem Cells Species: Human

We use this 'Human Troponin T (Cardiac) Antibody' for staining differentiated cardiac cells from non-muscle cells (iPSc, Fibroblasts, etc.).