Human Tryptase alpha /TPS1 Antibody

Catalog #: MAB2370 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2370
MAB2370-SP

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Human Tryptase alpha /TPS1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tryptase alpha /TPS1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50‑100% cross‑reactivity with recombinant human (rh) Tryptase beta 2 and no cross-reactivity with rhTryptase gamma 1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 274001

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase alpha /TPS1
Ile31-Pro275
Accession # AAA86934

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Tryptase alpha /TPS1

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha /TPS1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase alpha/TPS1

Tryptase alpha is a neutral serine protease that is enriched in mast cell granules. It has a unique Asp residue in the substrate-binding region and has either very restricted specificity or no protease activity at all.

Entrez Gene IDs

7177 (Human)

Alternate Names

alpha-Tryptase; EC:3.4.21.59; epididymis secretory sperm binding protein; mast cell alpha II tryptase; mast cell beta I tryptase; mast cell tryptase; TPS1; TPS2; TPSAB1; TPSB1; TPSB2; Tryptase 1; tryptase alpha II; Tryptase alpha; Tryptase alpha-1; tryptase beta I; tryptase beta-1; Tryptase beta-2; Tryptase I; tryptase II; tryptase-1; tryptase-2; tryptase-I; tryptase-III